PI3K特异性抑制剂对人大肠癌细胞HCT-8生长的影响  

Effect of PI3K inhibitor on proliferation of colorectal carcinoma cell line HCT-8

作　　者：王贵吉[1] 陈鹏涛[1] 裴迎新[2] 王相英 陈壬寅[4] 

机构地区：[1]郑州大学第一附属医院肿瘤科,郑州450052 [2]郑州大学公共卫生学院营养与食品卫生学教研室,郑州450001 [3]林州市人民医院内科,林州456550 [4]郑州大学第一附属医院病理科,郑州450052

出　　处：《郑州大学学报（医学版）》2008年第4期718-721,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:研究PI3K特异性抑制剂LY294002对人大肠癌细胞系HCT-8生长的影响。方法:将对数生长期的HCT-8细胞分组培养,对照组加入不含LY294002的培养液,实验1～4组加入LY294002,其终浓度分别为5、10、20和40μmol/L,培养24、48、72、96h后,MTT法测定细胞增殖抑制率。取传代培养并贴壁的HCT-8细胞分组培养,对照组常规培养,实验组培养液中加终浓度为10μmol/L的LY294002,培养24、48和72h后流式细胞仪检测细胞周期。取对数生长期的HCT-8细胞,对照组常规培养,实验组培养液中加终浓度为10μmol/L的LY294002,培养24h,培养前后,免疫细胞化学SP法检测P-Akt和NF-κB。结果:与对照组比较,随着LY294002剂量、作用时间的增加,对HCT-8细胞增殖抑制率增大(P<0.01)。10μmol/L的LY294002作用于HCT-8细胞,随着作用时间的延长,G0/G1期细胞逐渐增加,S和G2/M期细胞逐渐减少(P<0.05)。对照组培养前后HCT-8细胞P-Akt和NF-κB蛋白表达无明显变化,10μmol/L的LY294002作用后HCT-8细胞P-Akt和NF-κB蛋白表达降低(P<0.01)。结论:LY294002可能通过抑制P-Akt的表达,从而抑制人大肠癌HCT-8细胞的生长。Aim： To study the effect of LY294002 inhibitor on the proliferation of human colorectal cancer cell line HCT-8. Methods：The prohiferation inhibitory rate of HCT-8 cells treated by LY294002 at doses of 5,10,20,40 μmol/L for 24,48,72 and 96 h, respectively,was evaluated using MTT method. Cell cycle of HCT-8 cells treated by LY294002 at a doses of 10 μmol/L for 24,48, and 72 h, respectively, was detected by flow cytometry. The expression of P-Akt and NF-KB in HCT-8 cells treated by LY294002 at a dose of 10μmol/L for 24 h was detected by immunohistochemistry. HCT-8 cells treated without LY294002 were the control. Results： The proliferation of HCT-8 cells was inhibited in time- and dose-de- pendent manner by LY294002. After the treatment by 10 μmol/L LY294002 for 24 h,the proportion of HCF-8 cells in Go/ Gj phase was higher（P 〈 0.05 ） , that in S and G2/M phase was lower（P 〈 0.05 ）. The protein expression of P-Akt and NF- KB in HCT-8 cells was inhibited by LY294002（P 〈 0.01 ）. Conclusion： LY294002 can inhibit the expression of P-Akt of human colorectal cancer cell line HCT-8, and inhibit the proliferation of HCT-8.

关 键 词：PI3K/Akt LY294002 大肠癌 细胞周期 增殖 P-AKT NF-κB 

分 类 号：R735.3[医药卫生—肿瘤]