微生物来源抗肿瘤化合物SIPI-101-1的研究  

Studies on the Anti-tumor Agent SIPI-101-1 from an Microorganism

作　　者：张海红[1] 胡海峰[1] 张琴[1] 朱宝泉[1] 

机构地区：[1]上海医药工业研究院,上海200040

出　　处：《中国天然药物》2008年第4期316-318,共3页

基　　金：上海医药工业研究院创新基金资助(200605)~~

摘　　要：目的:分离链霉菌SIPI-101菌株生物合成抗肿瘤活性的化合物。方法:优化发酵条件,进行大规模发酵;发酵液经离心,菌丝体在碱性条件下经过甲醇提取、乙酸乙酯萃取、硅胶柱层析、结晶等步骤,分离获得活性化合物SIPI-101-1;并测定其质谱、紫外光谱、核磁共振图谱等数据,并研究其生物学活性。结果:通过理化性质和图谱数据的分析,确定化合物SIPI-101-1的化学结构;生物活性研究发现其具有抗肿瘤活性。结论:活性化合物SIPI-101-1属于吲哚咔唑类生物碱,与文献报道的staurosporine结构一致。AIM： To isolate anti-tumor agents from microorganism SIPI-101. MEHTODS： The fermentation condition of strain SIPI-101 was improved and scaled up. The mycelia were obtained by centrifugation. Using methanol and acetic ether extraction, silica gel column chromatography and crystallization, compound SIPI-101-1 was isolated from the broths; the spectral data of SIPI-101-1 were determined and its biological activity was studied. RESULT： The structure of SIPI-101-1 was identified by analyses of its physico-chemical properties, MS, UV and NMR spectra and anti-tumor activity was determined. CONCLUSION： The compound SIPI-101-1 produced by strain SIPI-101-1 is identical with staurosporine with strong anti-tumor activity.

关 键 词：抗肿瘤 吲哚咔唑类生物碱 链霉菌 发酵 

分 类 号：TQ465.92[化学工程—制药化工]