富马酸生产菌少根根霉的诱变筛选  

Mutagenisis and screening of high fumaric acid production Rhizopus arrhizus

作　　者：徐文雯[1] 马习东[1] 王芳[1] 谭天伟[1] 

机构地区：[1]北京化工大学生命科学与技术学院北京市生物加工过程重点试验室,北京100029

出　　处：《生物加工过程》2008年第4期61-64,共4页Chinese Journal of Bioprocess Engineering

基　　金：国家973计划资助项目(2007CB70780)

摘　　要：以实验室原少根根霉为出发菌种,通过紫外线和LiCl诱变处理,发现当紫外线照射时间为3 min,并在质量分数4%LiCl的平板中培养,可诱变出富马酸高产菌。利用溴甲酚绿加塑料小管的平板进行初筛,其生成的富马酸通过塑料小管底部渗透到指示培养基中,产生变色圈,根据变色圈大小可初步判断诱变株的产酸能力,大大缩短了筛选时间。在葡萄糖质量浓度为80 g/L时,诱变后的3#菌种在发酵72 h后能产生55.02 g/L的富马酸,比原菌种的富马酸产量提高了2.49倍。Fumaric acid over producting mutants was derived from rhizopus arrhizus by mutations with ultraviolet （UV） radiation and lithium chloride. The optimized condition of mutation was as follows ： UV irritation 3 rain; LiCI 4%. The mutants were directionally screened from the agar plates containing bromocresol green and plastic tube. According to the diameter of the color ring, high producticity rhizopus was quickly detected. This new method enormously enhanced the selection efficiency. When the glucose concentration was 80 g/L, the mutant Rhizopus arrhizus 3# after 72 h produced 55.02 g/L of fumaric acid, which was 2.49 times higher than that of the parent strain.

关 键 词：根霉 富马酸 筛选 

分 类 号：TQ921[轻工技术与工程—发酵工程]