子宫内膜癌p72、AIB1、erbB-2的表达及其对雌激素受体活性的协同作用  

Expression of Endometrial Cancer p72,AIB1,erbB-2 Detected by Real-time Quantitative RT-PCR and Coactivation to ERα

作　　者：赵琳[1] 范新[1] 王丽霞[1] 唐丽云[1] 姜立杰[1] 曲立兵[1] 平晓月[1] 

机构地区：[1]大连医科大学第二附属医院,辽宁大连116027

出　　处：《医学与哲学（B）》2008年第7期43-45,共3页Medicine & Philosophy(B)

摘　　要：为探讨p72、AIB1、erbB-2与雌激素受体(ERα)活性关系及意义,采用定量RT-PCR技术,检测不同分化程度的子宫内膜癌组织中ERa及其协同因子p72、AIB1、erbB-2的mRNA表达水平,并进行荧光素酶检测分析ERα活性与p72、AIB1、erbB-2表达的关系。结果显示,p72、AIB1和erbB-2的共同表达,促使雌激素受体的活性呈叠加增长。推测p72、AIB1与erbB-2对雌激素受体激活有相互促进作用。To determine how estrogens are involved in the growth of endometrial cancers we investigated the status of ERα and its cofactors p72 ,AIB1 and erbB--2 in endometrial cancers with varying degrees of differentiation by real--time RT--PCR,and their in vivo cellular effects on the transactivation function of ERa were examined by a transient expression assay. Results showed that the mRNA levels of p72,AIB1 and ERα decreased and those of erbB--2 increased with the loss of histological differentiation. Transient expression of p72 ,AIB1 and erbB--2 in human embryonic kidney 293T cells led to a synergistic promotion of the transactivation function of ERα in the presence of 17α--estradiol or 4--hydroxytamoxifen,an ERα AF--1 agonist/AF--2 antagonist,as a ligand. In conclusion, estrogen action through ERα AF--1 might be exerted by the increased expression of the coactivators,p72 and AIB1 ,together with cross talk between erbB--2 and p72 to accelerate the transactivation of ERa AF--1 in endometrial cancer.

关 键 词：子宫内膜癌 雌激素受体 协同因子 

分 类 号：R737.33[医药卫生—肿瘤]