从电鳐电器官中提取N_2-AchR纯品建立EAMG模型  

The model of EAMG was established by immunization with purified N_2-AchR extracted from Torpedo's electric organ

作　　者：杨颖 王静[2] 王旭丹[2] 王婉瑜[3] 

机构地区：[1]武警北京市总队医院,北京100027 [2]北京中医药大学基础医学院 [3]中国科学院昆明动物研究所

出　　处：《中国现代医药杂志》2008年第7期35-37,共3页Modern Medicine Journal of China

摘　　要：目的从厦门海域单鳍电鳐的电器官中提取烟碱型乙酰胆碱受体(N2-AchR)纯品,用纯化的N2-AchR免疫Lewis大鼠,建立实验性自身免疫性重症肌无力(EAMG)动物模型。方法参照文献报道[1-2]从电鳐电器官提取N2-AchR纯品,并采用SDS凝胶电泳蛋白定性鉴定及考马斯亮兰定蛋白含量;以纯提的蛋白主动免疫Lewis大鼠,共免疫3次,于末次免疫后第3日进行EAMG大鼠临床评分及攀网时间、肌电图、血清N2-AchRab含量、突触后膜上N2-AchR数目的检测。结果纯化的蛋白含量为1.097mg/ml(标准品为1mg/ml);EAMG模型组与佐剂组比较,攀网时间明显降低(P<0.01);临床评分及肌电图第五个反应幅度衰减百分率显著升高(P<0.01);血清N2-AchRab含量明显增加(P<0.01);突触后膜上N2-AchR数目显著减少(P<0.01)。结论从单鳍电鳐电器官纯提的N2-AchR蛋白成功诱导EAMG模型,为进一步研究重症肌无力创造了良好条件。Objective To study the rats model of experimental autoimmune myasthenia gravis （EAMG） established by immunization with puried N2-AchR extracted from Torpedo＇s electric organ. Methods According to references. purified N2-AchR protein was extracted from electric organ of torpedo,and identified with SDS gel electrophoresis.Lewis rats were immunized with the protein and completed Freund adjuvant（CFA） in EAMG group,and with CFA alone in CFA group for three times in general.After 22 days,the degree of clinical assessment of rats,repetitive nerve stimulation,serum level of anti- AchR antibody,AchR content of the post-synaptic membrane were obserbed. Results The content of the protein was 1.097mg/ml （standard：1mg/ml）. Compared with control rats, EAMG rats presented some symptoms at different degree like MG （P〈0.01） ;the percentage of decrement of the fifth wave and the levels of AchRab were higher markedly （P〈0.01） ;the numbers of AchR were lower obviously （P〈0.01）. Conclusion The model of EAMG established by immunization with purified N2-AchR extracted from Torpedo＇s electric organ could provide better condition for the study of MG.

关 键 词：实验性自身免疫性重症肌无力 烟碱型乙酰胆碱受体 电鳐 

分 类 号：R-332[医药卫生] R746.1