紫外诱变选育高效微生物絮凝剂产生菌及其应用  

Mutation breeding of bioflocculant-producing strain with high-efficiency by ultraviolet and its application

作　　者：吴焕利[1] 冯贵颖[1] 赵淑艳[2] 张雪峰[1] 杨娜[1] 

机构地区：[1]西北农林科技大学污染控制研究中心,陕西杨凌712100 [2]西藏农牧学院资源环境系,西藏林芝860000

出　　处：《西北农林科技大学学报（自然科学版）》2008年第8期113-118,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：2006年第一批中央环境保护专项基金(财建[2006]859号)

摘　　要：【目的】为紫外诱变技术在环境工程中的应用提供参考。【方法】由1株絮凝剂产生菌XL-18出发,经过紫外诱变得到絮凝活性高的正突变菌株YB-1,用红外光谱、薄层色谱分析纯化后YB-1产微生物絮凝剂的成分,苯酚-硫酸法分析样品中糖含量,双缩脲法分析样品中蛋白质含量,并用YB-1产微生物絮凝剂处理水性油墨废水,研究了不同因素(pH、投加量、助凝剂)对絮凝效果的影响。【结果】菌株YB-1的絮凝率为95.0%;YB-1产微生物絮凝剂含有羟基、羧基等官能团,总糖含量为6.630 g/L,不含蛋白质,其是由葡萄糖和甘露糖为主要单体组成的多糖;添加0.5 mol/L CaCl2溶液作助凝剂、废水pH 8.0、YB-1发酵液投加量为60 ml/L时,水性油墨废水的COD、浊度、色度去除率分别为88.25%,94.55%和95.27%,并得到了该微生物絮凝剂的动力学方程。【结论】紫外诱变技术可以明显提高微生物絮凝剂的絮凝活性,是获得高效微生物絮凝剂产生菌的有效途径。[Objective] The study was done to provide reference for the application of UV induced mutation technology in environmental engineering. [Method] A high efficient bioflocculant strain YB-1 has been obtained by UV induced mutation from an inefficient strain named XL-18. The bioflocculant element was obtained with TLC and analyzed with IR. The sugar content and protein content were analyzed with Phenol-sulfate quantitative analysis and Biuret law. The wastewater of water-based ink was treated by YB- 1 to investigate the effects of pH, dose and coagulantaids on flocculation. [Result] The flocculant efficiency of YB-1 was 95 %. The IR spectrum showed that flocculant YB-1 contains hydroxyl,carboxyl etc. functional groups,total sugar content 6. 630 g/L, no proteins. The flocculant consisted of mannose and glucose,as well as other monomers. The removal rate of COD,turbidity and chroma were 88.25% ,94.25% and 95.27 respectively,when pH was 8.0; inoculum was 60 ml/L and 0.5 mol/L CaCl2 as coagulant aid. In addition, the dynamics equation of deposition was deduced. [Conclusion] UV induced mutation can improve flocculation activity significantly. It is an effective way to breed bioflocculant-producing strain with high efficiency.

关 键 词：紫外诱变 微生物絮凝剂 水性油墨废水 动力学方程 

分 类 号：X172[环境科学与工程—环境科学] X703