胚胎神经上皮干细胞体外克隆增殖与分化的实验研究  

THE AMPLIFICATION AND DIFFERENTIATION OF THE EMBRYONIC NEUROEPITHELIAL STEM CELLS IN VITRO

作　　者：王家增 薛良华 赵遵杭 孙晋浩[2] 

机构地区：[1]山东医学高等专科学校,山东临沂276002 [2]山东大学医学院人体解剖学教研室,山东济南250012

出　　处：《山东医学高等专科学校学报》2008年第3期166-168,F0003,共4页Journal of Shandong Medical College

基　　金：山东省优秀中青年科学家科研奖励基金(No.2004BS02012)

摘　　要：目的观察胚胎神经上皮干细胞在体外克隆增殖和诱导分化的情况。方法取孕11 d大鼠的胚胎,经分离获得神经管上皮细胞,在无血清培养基内克隆、增殖、传代。取不同时期的神经球进行神经干细胞的鉴定和诱导分化,并通过免疫组织化学染色鉴定分化的结果。结果胚胎神经上皮干细胞在体外能够克隆、增殖,形成的神经球呈nestin阳性,在分化培养基内可分化为星型胶质细胞和神经元。结论胚胎神经上皮干细胞可在体外进行单细胞克隆和诱导分化。Objective To observe the amplification and differentiation of the embryonic neuroepithelial stem cells in vitro. Methods The neuroepithelial stem cells were obtained from the E11d embryos,and cultured in the free-serum medium： These cells were identified and induced during different culturing periods in vitro by immunocytochemistry. Results The embryonic neuroepithelial stem cells could amplify through cloning and the neurospheres were nestin-positive. The neurons and astrocytes were observed in the differentiation medium. Conclusion The embryonic neuroepithelial stem cells can grow and amplify by single cloning and differention by inducing in vitro.

关 键 词：胚胎 神经上皮 干细胞 增殖 分化 

分 类 号：Q813.1[生物学—生物工程]