从分子系统发育探讨内生拟盘多毛孢与病原拟盘多毛孢的关系  

Molecular phylogenetic investigation on relationship of endophytic and pathogenic Pestalotiopsis species

作　　者：韦继光[1] 徐同[2] 黄伟华[1] 郭良栋[3] 潘秀湖 

机构地区：[1]广西大学农学院,广西南宁530005 [2]浙江大学农业与生物技术学院生物技术研究所,浙江杭州310029 [3]中国科学院微生物研究所真菌地衣系统学重点实验室,北京100101 [4]广西高峰林场,广西南宁530001

出　　处：《浙江大学学报（农业与生命科学版）》2008年第4期367-373,共7页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目(3027001530470004);广西大学科研基金资助项目(X051098)

摘　　要：收集马尾松、罗汉松、竹柏、山茶、茶和茶梅等植物的内生和病原拟盘多毛孢菌株,进行形态鉴定,根据rDNA ITS序列分析建立的系统发育树探讨病原与内生拟盘多毛孢之间的相互关系.结果表明,同一个种拟盘多毛孢不论是内生还是病原均聚在系统发育树的同一个分支,如罗氏拟盘多毛孢(Pestalotiopsis lawsoniae)既是马尾松枝条的内生真菌,又是马尾松针叶的病原真菌;卡斯特尼拟盘多毛孢(Pestalotiopsis karstenii)是山茶枝条的内生真菌,在山茶叶片上也是病原真菌;石楠拟盘多毛孢(Pestalotiopsis photiniae)是山茶和茶梅枝条的内生真菌,在马尾松针叶上也是病原真菌;茶拟盘多毛孢(Pestalotiopsis theae)是茶的病原真菌,在金花茶枝叶和罗汉松枝上是内生真菌.说明某些拟盘多毛孢在不同(或相同)的植物或器官中可以表现为内生状态或病原状态,内生拟盘多毛孢在一定条件下可以转变为病原拟盘多毛孢.本研究结果还支持拟盘多毛孢种一般对寄主植物没有专化性的结论.Isolates of endophytic and pathogenic Pestalotiopsis from Pinus massoniana, Podocarpus macrophyllus, Podocarpus nagi, Camellia japonica, Camellia sinensis and Camellia sasanqua were collected and morphologically identified. The investigation on relationship between endophytic Pestalotiopsis and pathogenic Pestalotiopsis species based on molecular phylogenetic tree of rDNA ITS sequences was carried out. It was found that Pestalotiopsis isolates of the same species no matter it was pathogenic or endophytic were clustered in the same branch of phylogenetic tree, for example, P. lawsoniae was an endophytic fungus on twigs and a pathogen on leaves of P. massoniana ; P. karstenli was an endophytic fungus on twigs and a pathogen on leaves of C. japonica ; P. photiniae was an endophytic fungus on twigs of C. japonica and C. sasanqua and a pathogen on leaves of P. massoniana ; and P. theae was a pathogen on leaves of C. sinensis and an endophytic fungus on twigs and leaves of C. nitidissima and twigs of P. macrophyllus. The result showes that some Pestalotiopsis species could be endophytic and pathogenic states on different species or parts of plant and an endophytic Pestalotiopsis could transformed into a pathogenic Pestalotiopsis under a certain condition. The result also supportes the previous conclusion that Pestalotiopsis species are not generally specific to host plants.

关 键 词：内生拟盘多毛孢 病原拟盘多毛孢 分子系统发育分析 

分 类 号：Q949.329[生物学—植物学]