竹材木质素选择性降解菌株的分子鉴定  

Molecular identification of the strain ZNLD-18 selectively decomposing bamboo lignin

作　　者：周国英[1] 李河[1] 

机构地区：[1]中南林业科技大学资源与环境学院,湖南长沙410004

出　　处：《浙江林学院学报》2008年第4期497-501,共5页Journal of Zhejiang Forestry College

基　　金：湖南省自然科学基金资助项目(04jj3055)

摘　　要：鉴定1株从腐竹中筛选的高效选择性降解竹材木质素的优良菌株ZNLD-18,为生物法提取可纺性竹原纤维奠定基础。利用真菌通用引物ITS 1和ITS 4扩增并测定菌株的rDNA ITS区序列,序列总长541 bp。把所得序列在GenBank中进行Blast搜索得到同源性较高的同属不同种菌株序列。比较这些序列的遗传距离,显示菌株ZNLD-18与Trametes versicolor的ITS区序列同源性为99%以上。利用PAUP软件构建分子系统发育树,通过对该树的分析并综合菌株的形态特征,鉴定菌株ZNLD-18为白腐菌Trametes versicolor。This objective was to provide basis for the biological extracting of the spinning bamboo fiber. The strain ZNLD-18, separated from decaying bamboo, which was able to decompose selectively the bamboo lignin, was identified by polymerase chain reaction （PCR）. The rDNA ITS sequences of the strain were amplified and tested by the fungi universal primers ITS1 and ITS4. The sequence length was 541 base pair （bp）. The sequences were Blast searched within the GenBank to obtain the different strains＇ sequences with the similar homology. The comparison of the genetic distances of these sequences showed that the ITS sequences of ZNLD-18 and Trametes versicolor were with over 99% homology. A molecular phylogenetic tree was built with Phylogenetic Analysis Using Parsimony （PAUP） software. Given the analysis results of the tree and the morphological characteristics of the strain, ZNLD-18 was Trametes versicolor.

关 键 词：微生物学 竹材 木质素 选择性降解 菌株 RDNA ITS 分子鉴定 

分 类 号：Q949.329[生物学—植物学]