紫芝发酵液中靛红的分离纯化与结构鉴定  

Isolation and Identification of Isatin from the Fermentation Broth of Ganoderma sinensis

作　　者：桑红娇[1] 张琴[1] 胡海峰[1] 朱宝泉[1] 

机构地区：[1]上海医药工业研究院生物制药部,上海200040

出　　处：《食用菌学报》2008年第2期61-63,共3页Acta Edulis Fungi

摘　　要：紫芝发酵液经离心、乙酸乙酯萃取后进行硅胶柱层析,采用环己烷-乙酸乙酯和乙酸乙酯-乙醇系统梯度洗脱并结晶得化合物LZ-1。通过对LZ-1的紫外、质谱和核磁共振等波谱数据进行分析,结合文献对照确定该化合物的结构为吲哚-2,3-二酮,即靛红。Concentrated Ganoderma sinensis fermentation broths were extracted with ethyl acetate. Extracts were applied to a silica gel column and eluted with cyclohexane-ethyl acetate and with ethyl acetate-ethanol. The crystallized product, Compound LZ-1, was identified as indole-2, 3-dione （isatin） based on UV, MS and NMR spectral data.

关 键 词：紫芝 靛红 吲哚 发酵液 

分 类 号：R284[医药卫生—中药学]