大麦黄花叶病毒单克隆抗体的制备  

PREPARATION OF MONOCLONAL ANTIBODIES TO BARLEY YELLOW MOSAIC VIRUS

作　　者：赵小立[1] 马红[1] 翁醒华[1] 徐阿炳[1] 黄纯农[1] 

机构地区：[1]杭州大学生命科学学院,杭州310028

出　　处：《微生物学报》1997年第5期401-404,共4页Acta Microbiologica Sinica

基　　金：浙江省"八五"攻关项目并得到亚洲生物技术和生物多样性项目的资助

摘　　要：大麦黄花叶病毒(Barley yellow mosaic virus,BaYMV)广泛分布于西欧、日本和我国长江中下游和东部沿海地区,严重危害了大麦生产,并呈现出不断蔓延扩大的趋势。该病毒经土壤禾谷多粘菌介体感染大麦后,病毒繁殖发病的状况,易受到大麦品种不同、环境温湿度等因素的影响,发病程度的差异很大,而且还发现带毒隐症的大麦品种,使大麦抗源筛选和大麦抗性品种的选育工作受到严重干扰,因此迫切需要建立一种快速、灵敏和准确的检测方法。目前,比较理想的方法是ELISA等免疫学技术。Using hybridoma techniques, six hybridoma cell lines: 4D6, 4D4, 4F10, 3H6, 3H8 and 4E9 secreting monoclonal antibodies to Shanghai Isolate of Barley yellow mosaic virus (BaYMV) were prepared by fusion between myeloma cells NS-1 and spleen cells of BALB/ C mouse immunized with BaYMV. The subclasses of monoclonal antibodies obtained belong to IgG2a. The cross-reaction test with different viruses (SMV, TMV and three isolates of TuMV) showed that six monoclonal antibodies were specific to BaYMV. The titers of six monoclonal antibodies to BaYMV in their cell cultured supematauts were l:1-5×104, and that of 4F10 in ascitic fluid was 1:6.4 × 105 by using indirected ELISA test respectively.

关 键 词：大麦 黄花叶病毒 单克隆抗体 制备 

分 类 号：S435.123[农业科学—农业昆虫与害虫防治] Q939.91[农业科学—植物保护]