乳杆菌DM8909对大肠杆菌粘附阴道上皮细胞抑制作用的研究  

INHIBITION OF ADHESION OF ESCHERICHIA COLI TO HUMAN VAGINAL EPITHELIAL CELLS BY LACTOBACILLUS DM8909 STRAIN IN VITRO

作　　者：蒋寒青[1] 易庆[1] 康白[1] 朴永哲[2] 祝元丽 

机构地区：[1]大连医科大学微生态学研究所 [2]大连医科大学科达药业有限公司

出　　处：《中国微生态学杂志》1997年第5期18-19,共2页Chinese Journal of Microecology

摘　　要：本文对乳杆菌ＤＭ８９０９对大肠杆菌粘附阴道上皮细胞的抑制作用进行了研究。采用３Ｈ－ＴｄＲ将由阴道分离的大肠杆菌进行同位素标记。刮取健康妇女阴道上皮细胞与其进行体外粘附试验作为对照组，试验组先行阴道上皮细胞与乳杆菌ＤＭ８９０９粘附后，再加入标记的大肠杆菌。结果显示：试验组阴道上皮细胞粘附的大肠杆菌的ｃｐｍ值明显低于对照组（Ｐ＜０．０５），说明乳杆菌ＤＭ８９０９能有效地抑制大肠杆菌对阴道上皮细胞的粘附。This study investigated the effect of Lactobacillus DM8909 strain on the adhesion of E.Coli to normal human vaginal epithelial cells by in vitro methods. Lactobacillus DM8909 strain was isolated from vagina of a health young woman and identified as Lactobacillus debrueckii lactis. Vaginal epithelial cells,collected from health volunteers,were suspended immediately in RPMI-1640 medium and adjusted to 14×105 cells/ml.E.coli was vaginal isolate and labled 3 H-TdR.by inoculating it in Nb fluid culture containing 20uci/ml 3 H-TdR. Labled E.coli were suspended in RPMI-1640 on the concentration of 108/ml.Lactobacillus DM8909 cultures were also prepared into suspension of 108/ml in RPMI1640. Results showed that cpm of the experimental group was significantly lower than that of the control group(P<005). It was suggested that Lactobacillus DM8909 strain could effectively inhibit the adherence of E.coli to human vaginal epithelial cells. Lactobacillus Adhesion E.coli Vaginal epithelial cell

关 键 词：乳杆菌 粘附 大肠杆菌 阴道上皮细胞 阴道炎 

分 类 号：Q939.110.5[生物学—微生物学] R711.31[医药卫生—妇产科学]