原位核酸杂交检测宫颈癌中金黄色葡萄球菌L型DNA  

DETECTION OF STAPHYLOCOCCUS AUREUS L-FORMS DNA IN CERVICAL CARCINOMA TISSUES USING IN SITU HYBRIDIZATION

作　　者：何杰[1] 张世馥[1] 姚敏[1] 李明君[1] 汪万英[2] 崔怀波 

机构地区：[1]蚌埠医学院病理教研室 [2]蚌埠医学院附属医院病理科 [3]蚌埠医学院附属医院肿瘤外科 [4]蚌埠医学院微生物教研室

出　　处：《中国微生态学杂志》1997年第5期41-44,共4页Chinese Journal of Microecology

摘　　要：应用病原微生物培养、改良革兰氏染色、免疫组化染色及原位核酸杂交等方法，对５０例宫颈癌和３３例慢性宫颈炎进行了研究。结果发现宫颈癌病原微生物培养细菌及其Ｌ型阳性感染率为５８％（２９／５０例），以金黄色葡萄球菌（金葡菌）及其Ｌ型多见（１３／２９例）；宫颈癌和慢性宫颈炎组织切片革兰氏染色，细菌Ｌ型检出率分别为６６％和４５．５％；免疫组化染色（ＡＢＣ法）金葡菌Ｌ型抗原阳性率各为６８％和４８．５％。Ｌ型主要分布于癌间质、癌巢及宫颈上皮下结缔组织内。１２例宫颈癌金葡菌Ｌ型ＤＮＡ探针原位杂交显示，１０／１２例癌细胞核内有金葡菌Ｌ型ＤＮＡ杂交阳性信号，表明金葡菌Ｌ型ＤＮＡ已进入宫颈癌细胞内，可能会在基因水平上影响宫颈上皮恶变，提示宫颈癌发生与金葡菌Ｌ型感染关系密切，值得进一步研究。Fifty specimens of carvical carcinomas and thirty three chronic cervicitis were taken from biopses and surgical excision were collected for isolation of pathogenic organisms, immunohis to chemical technigue (ABC) and in situ hybridization with for staphy lococcus aureus L-forms DNA. There results suggested that the 29 cases (58%) of tissues culture of cervical carcinomas appeared to be associated with both bacteria and L-forms, staphylococcus aureus L-forms of which were mainly (44.83%),x2=10445,P<0.01。In 50 cases of cervical carcinomas and 33 chronic cervicitis, the detected rates of L-forms were present in 66%, 45.5% respectively, the positive rate of staphy lococcus aureus cowan I L-forms antigens were 68% and 48.5%, respectively, the result of bacterial DNA in situ hybridization showed that 10/12 (83.3%) cervical carcinomas ccurred in association with L-forms DNA, mostly localized with in the nucleus. It has been suggested that there is a close relationship between infection of staphylococcus aureus L-forms and cervical carcinomas. L-forms may be caused gene mutatin.

关 键 词：子宫颈肿瘤 癌 原位核酸杂交 金黄色葡萄球菌 

分 类 号：R737.330.2[医药卫生—肿瘤]