黄芪有效部位群对大鼠肌成纤维细胞增殖移行及胞内Smads蛋白磷酸化的影响  

The effects of effective fractions of astragalus (EFA) on the proliferation and migration and Smad proteins phosphorylation in rat myofibroblast

作　　者：何淑芳[1] 梁佳玉[1] 方佳[1] 杨林[1] 杨雁[1] 张学军[2] 

机构地区：[1]安徽医科大学药理学教研室 [2]安徽医科大学皮肤病研究所,安徽医科大学第一附属医院皮肤性病科

出　　处：《中国药理学通报》2008年第7期885-888,共4页Chinese Pharmacological Bulletin

基　　金：中国博士后科学基金资助项目(No20060400716);中国教育部留学回国人员科研启动基金资助项目(No20070024);安徽省教育厅自然科学基金资助项目(NoKJ2008A30ZC);安徽医科大学人才基金资助项目

摘　　要：目的研究黄芪有效部位群（effective fractions of astragalus，EFA）对转化生长因子-β1（transforming growth factorbeta1，TGF-β1）诱导的大鼠肌成纤维细胞（myofibroblast，MFB）增殖、移行及胞内Smads蛋白磷酸化的影响。方法采用MTT法观察EFA不同浓度对新生小牛血清（NBS）或TGF-β1诱导的MFB细胞增殖的影响；浸润小室法观察EFA对TGF-β1诱导的MFB细胞移行的影响；免疫吸附和Westem blot法研究EFA对TGF-β1诱导的MFB细胞内Smads蛋白磷酸化的影响。结果EFA（10-160mg·L^-1）呈浓度依赖性抑制NBS及TGF-βl诱导的MFB细胞增殖；EFA（80mg·L^-1）能明显抑制TGF-β1诱导的MFB细胞移行；EFA（10-80mg·L^-1）呈浓度依赖性抑制TGF-β1诱导的MFB细胞内Smad2C、Smad2L蛋白磷酸化，但各组间Smad2／3蛋白表达水平无差别。结论EFA通过干扰TGF-β／Smad信号转导通路而抑制MFB细胞的增殖、移行从而起到抗肝纤维化的作用。Aim To investigate the effects of EFA on the proliferation, migration and Smad proteins phosphorylation in rat myofibroblast induced by TGF-β1. Methods The effects of EFA on the proliferation of MFB cells induced by NBS and/or TGF-β1 were detected by MTr method. The effects of EFA on the migration of MFB cells induced by TGF-β1 were assayed with the use of Chemotaxicell chamber. Immunoprecipitation and Western blot methods were used to investigate the effects of EFA on the expression levels of pSmad2C, pSmad2L and Smad2/3 in MFB cells activated by TGF-β1. Results EFA （ 10-160 mg·L^-1 ） inhibited the proliferation of MFB cells induced by NBS and/or TGF-β1 in a dose-dependent manner. The migration of MFB cells induced by TGF-β1 was reduced by EFA at the dose of 80 mg·L^-l. EFA （10 -80 mg·L^-l） inhibited the expression of pSmad2C,pSmad2L in MFB cells induced by TGF-β1 in a dose-dependent manner, but significant difference in the expression of Smad2/3 was not observed among each group. Conclusion EFA could inhibit the proliferation and migration by interfering with TGF-β/Smad signal transduction in MFB cells,which might be the mechanism of anti-hepatic fibrotic effects of EFA.

关 键 词：黄芪有效部位群 肌成纤维细胞 转化生长因子-β1 SMAD 

分 类 号：R-332[医药卫生] R284.1