HYAL1基因过表达对乳腺癌细胞迁移能力和血管生成能力及增殖的影响  

Effects of HYAL1 gene overexpression on invasive,angiogenic and proliferative ability of breast cancer cells

作　　者：苏新良[1] 任国胜[1] 王小毅[1] 谭金祥[1] 

机构地区：[1]重庆医科大学附属第一医院内分泌外科,重庆400016

出　　处：《第三军医大学学报》2008年第15期1470-1472,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30070292)~~

摘　　要：目的探讨透明质酸酶HYAL1基因过表达对人乳腺癌细胞株MCF-7和ZR-75-30的迁移能力和血管生成能力以及增殖的影响。方法应用双室联合培养技术,建立体外肿瘤侵袭和血管生成模型,观察过表达HYAL1基因对乳腺癌细胞穿透ECM gel和诱导血管内皮细胞形成血管能力的影响;应用MTT和流式细胞术检测细胞的增殖能力变化。结果过表达HYAL1基因的乳腺癌细胞MCF-7和ZR-75-30(实验组)穿透ECM的能力以及诱导血管生成的能力均强于对照组(P<0.05);但是,过表达HYAL1基因的乳腺癌细胞增殖能力与对照组比较没有明显差异(P>0.05)。结论过表达HYAL1基因能促进人乳腺癌细胞在体外侵袭和诱导血管生成,但是对乳腺癌细胞的增殖没有影响。Objective To study the effects of HYAL1 gene overexpression on invasive, angiogenic and proliferative ability of breast cancer cell lines MCF-7 and ZH-75-30. Methods Double-chamber co-culture technique was applied to construct the invasive model and angiogenic model in vitro, which was used to detect the invasive and angiogenic potential of breast cancer cell ; MTr and flow cytometry were used to detect the proliferation of breast cancer cells. Results Breast cancer cells overexpressing HYAL1 gene showed stronger invasive potential and angiogenic potential than control cells, but had no significant difference on proliferative potential. Conclusion Overexpression of HYAL1 gene can promote the invasion and angiogenesis of breast cancer cells in vitro, but not affect the proliferation.

关 键 词：乳腺癌 透明质酸酶 侵袭 血管生成 增殖 

分 类 号：R73-37[医药卫生—肿瘤] R730.23[医药卫生—临床医学]