动态张、压应力刺激下人牙周膜成纤维细胞细胞骨架变化  

Change of cytoskeleton by dynamic tensile and compressive stress in human periodontal ligament fibroblasts

作　　者：朱庆党[1] 巢永烈[2] 陈新民[2] 杨艳丽[1] 

机构地区：[1]浙江大学医学院附属口腔医院修复科,310006 [2]四川大学华西口腔医学院修复科

出　　处：《实用口腔医学杂志》2008年第4期559-562,共4页Journal of Practical Stomatology

基　　金：国家自然基金资助(编号:30700959);浙江省医药卫生科学研究基金资助项目(编号:2008A113)

摘　　要：目的:观察不同动态张、压应力刺激下人牙周膜成纤维细胞细胞骨架的变化。方法:用Forcel四点弯曲加载装置对体外培养的人牙周膜成纤维细胞分别施加不同动态的张、压应力(强度为1000、2000、4000μstrain,加力时间为0、1、2、4、8、12h),经荧光倒置显微镜观察施加不同动态张、压应力后细胞形态变化,细胞骨架荧光染色强度测定分析细胞骨架F-actin表达量的变化。结果:加力后细胞骨架形态和微丝蛋白发生规律性变化;细胞F-actin荧光染色强度正常→下降→正常;细胞骨架对张、压应力作用的反应敏感程度无明显差异。结论:在一定的张、压应力范围内人牙周膜成纤维细胞细胞骨架的形态结构具有一定的稳定性。Objective： To investigate the effects of different kinds of mechanical forces on the changes of cytoskeleton in cultured human periodontal ligament fibroblasts （hPDLFs）. Methods： A new cyclic strain loading apparatus ＂Forcel＂, a four points bending device, was used for mechanically loading. Cells were loaded by three levels （1 000, 2 000, 4 000 ustrain） of tensional and compressive forces for different times（0, l, 2, 4, 8, 12 h） after strain loading and data were collected. The morphological changes of cytoskeleton were observed by fluorescence converted microscope. Fluorescence intensity of F-actin was measured with image-analysis system at different time points after loading mechanical forces. Results： The changes of morphological characteristics of cytoskeleton and fluorescence intensity of F-actin were statistically significant. After being loaded, the shape of cells became unregular, cell arranged along the direction of loaded forces, microfilament became thinner and arrange unevenly, and fluorescence intensity of F-actin became weaker. As the effect of mechanical forces became longer, the above changes vanished. No significant difference between tension and compression can be observed. Conclusion： Dynamic mechanical forces can change the morphological characteristics of cytoskeleton and fluorescence intensity of F-actin. To a certain extent of mechanical stimuli the cytoskelelon of hPDLFs is stable, the structures of hPDLF have stability and fitting mechanical stimuli can promote their function.

关 键 词：人牙周膜成纤维细胞 张应力 压应力 细胞骨架 

分 类 号：R78[医药卫生—口腔医学]