α1,3 FucT-Ⅶ基因转染对人结肠癌LOVO细胞的作用  

The effects of α1,3 FucT-Ⅶ gene transfection on human colon cancer cell line LOVO

作　　者：李文桦[1] 罗建民[2] 张英[3] 张文[1] 

机构地区：[1]复旦大学附属肿瘤医院肿瘤内科,上海200032 [2]复旦大学附属肿瘤医院肿瘤内科中心实验室,上海200032 [3]复旦大学上海医学院生物化学与分子生物学系-卫生部糖复合物重点实验室,上海200032

出　　处：《复旦学报（医学版）》2008年第4期569-573,共5页Fudan University Journal of Medical Sciences

基　　金：复旦大学基础-临床医学交叉研究基金资助

摘　　要：目的观察α1,3 FucT-Ⅶ基因转染对人LOVO细胞的生物学行为的改变。方法采用RT-PCR和Western blot方法检测转染前后LOVO细胞中α1,3 FucT-Ⅶ基因的表达,CCK-8法检测细胞增殖,流式细胞术分析细胞周期,并通过体外迁移、侵袭实验检测转染前后LOVO细胞运动侵袭能力的变化。结果LOVO细胞微弱表达α1,3 FucT-Ⅶ,RT-PCR和Western blot方法验证转染后的LOVO/FucT-Ⅶ高表达α1,3 FucT-Ⅶ。转染后细胞生长增快,S期细胞增多而G1期细胞减少,细胞趋化性迁移和侵袭能力增强。结论α1,3 FucT-Ⅶ基因能改变人结肠癌LOVO细胞的生物学行为,增强细胞趋化性迁移和侵袭等转移表型。Objective To investigate the effect of α1,3 FucT-Ⅶ gene transfection on human colon cancer cell line LOVO. Methods Expression of the α1,3 FucT-Ⅶ mRNA and protein was measured by RT-PCR and Western blot. Cell proliferation was measured by CCK-8 assay. Cell cycle was analyzed by flow cytometry. The alteration of LOVO cells metastatic potential before and after α1,3 FucT-Ⅶ gene transfection were assayed by cell chemotactic migration and invasion. Results α1,3 FucT-Ⅶ was weakly expressed in human colon cancer cell line LOVO. The expression of α1,3 FucT-Ⅶ in transfected LOVO cells was detected by RT-PCR and Western blot methods. The proliferation of cells was increased after transfection with more cells in S period and less in G1 period. The cell chemotactic migration and invasion were enhanced after α1,3 FucT-Ⅶ gene transfection. Conclusions α1,3 FucT-Ⅶ gene promotes cell proliferation and metastatic potential of LOVO cells.

关 键 词：结直肠癌 α1 3 FucT-Ⅶ基因 糖基转移酶 

分 类 号：R735.35[医药卫生—肿瘤] R735.7[医药卫生—临床医学]