LPS和PMA对人眼晶状体上皮细胞增殖和EGFR表达的影响  

Effects of LPS and PMA on proliferation of human len epithelial cells and expression of epidermal growth factor receptor in human len epithelial cells

作　　者：王哲文[1] 施小茹[1] 李婷钰[1] 周世平[1] 张红[1] 

机构地区：[1]吉林大学第一医院眼科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2008年第4期665-667,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅基金资助课题(200505185)

摘　　要：目的:观察人晶状体上皮(HLE)细胞表皮生长因子受体(EGFR)的表达,探讨脂多糖(LPS)和佛波醇酯(PMA)对HLE细胞增殖及EGFR表达的影响。方法:应用免疫组织化学方法检测年龄相关性白内障的HLE细胞组织及培养的胎儿HLE细胞EGFR蛋白表达,RT-PCR方法检测EGFR mRNA表达;0.5、1.0、2.0mg·L-1的LPS及25、50、100nmol·L-1PMA作用培养的HLE细胞48h后,MTT法检测细胞增殖率,RT-PCR方法检测EGFR mRNA表达。结果:免疫组织化学和RT-PCR检测结果显示,年龄相关性白内障的HLE细胞及培养的胎儿HLE细胞中EGFR蛋白及mRNA呈阳性表达;0.5、1.0和2.0mg·L-1LPS对HLE细胞的增殖率分别为(3.21±0.42)%、(12.25±1.34)%和(36.67±3.65)%,2.0mg·L-1LPS组与其他两组比较差异有显著性(F=7.709,P<0.01),并使EGFR mRNA表达增加;25、50和100nmol·L-1PMA各组组间比较,PMA对HLE细胞的增殖率差异无显著性(P>0.05),对EGFR mRNA表达无影响。结论:LPS通过促进EGFR表达使HLE细胞增殖从而促进后发性白内障(PCO)的发生。Objective To observe the effects of LPS and PMA on proliferation of human len epithelial （ HLE ） cells and expression of epidermal growth factor receptor （EGFR） in HLE cells. Methods The expressions of EGFR protein of HLE cells from felus, adult lens age-related cataract and cultured HLE cells were detected by immunohistochemical staining. The expression of EGFR mRNA was detected by RT-PCR. The effects of LPS （0.5, 1.0, 2.0 mg · L^-1） and PMA （25, 50, 100 nmol · L^-1） on proliferation of HLE cells were detected by MTT colorimetry method, and the EGFR mRNA expression in HLE cells was determined by RT-PCR. Results The expressions of EGFR protein and mRNA were positive in HLE cells from felus, adult lens age-related cataract and cultured HLE cells. The proliferation rates of HLE cells treated with 0.5, 1.0, 2.0 mg · L^-1 LPS were （3.21±0.42）%, （12.25±1.34）% and （36.67±3.65）%, respectively. The proliferation rate of HLE cells in 2.0 mg · L^-1 LPSgroup was higher than those in0.5 and 1.0 mg · L^-1 LPS groups （F=7.709, P〈0.01） . 2.0 mg · L^-1 LPS increased the expression of EGFR mRNA. There was no significant difference in proliferation rate between25, 50and100 nmol · L^-1PMA groups （P〉0.05）. PMA （25, 50, 100 nmol · L^-1） could not effect the expression of EGFR mRNA in HLE cells . Conclusion Inflammation stimulant factor such as LPS can promote the proliferation of HLE cells by increasing the expression of EGFR and result in occurrence of posterior capsular opacifition （PCO）.

关 键 词：人晶状体上皮细胞 表皮生长因子受体 脂多糖 佛波醇酯 

分 类 号：Q813[生物学—生物工程] R746.1[医药卫生—神经病学与精神病学]