Quercetin治疗裸鼠移植人肝癌的作用及机制  

Effect and mechanism of Querecetin treatment of transplanted hepatic cancer in nude mice

作　　者：黄熙[1] 张继红[2] 

机构地区：[1]北京大学深圳医院肝胆外科,广东深圳518036 [2]广东药学院临床医学院

出　　处：《中国普通外科杂志》2008年第7期663-667,共5页China Journal of General Surgery

基　　金：广东省深圳市科技计划项目资助课题(200602054);广东省医学科学基金立项课题(A2004537)

摘　　要：目的探讨quercetin治疗裸鼠移植人肝癌中的作用及三磷酸肌醇(IP3)和Bax基因表达变化。方法采用quercetin治疗实验性肝癌,观察肝癌增长情况;用同位素试剂盒检测肝癌组织IP3含量;RT-PCR分析癌组织bax mRNA的表达;Western blotting分析肝癌组织bax蛋白的表达。结果治疗组肝癌体积和重量均显著低于对照组[体积(15.8±10.1)mm3vs.(52.3±26.5)mm3;重量(44.8±10.4)mgvs.(91.3±31.4)mg;均P<0.01],IP3含量显著低于对照组[(15.9±2.8)pmol/mg vs.(35.3±6.6)pmol/mg;均P<0.01],bax mRNA表达与对照组无显著性差异[RI(灰度与面积之积的相对强度)(0.64±0.12)vs.(0.56±0.15),P>0.05],但bax蛋白表达显著高于对照组[RI(3.16±0.95)vs.(1.37±0.48)]。结论结论Quercetin能减少IP3生成,上调肝癌组织bax蛋白的表达,抑制裸鼠移植人肝癌的增长。Objective To study the role of 1 , 4, 5-trisphosphate inositol （IP3） and bax gene expression in inhibiting transplanted hepatocellular carcinoma of nude mice by quercetin. Methods After animals with hepatocellular carcinoma were treated with quercetin 1 mg/kg/d （ip） for 3 weeks, the volume and weight of tumor was measured, and IP3, Bax mRNA, and Bax protein were assayed by IP3 - [ 3 H ] Birtrak assay, RT-PCR, and Western blotting, respectively; and compard with coutrol group. Results The tumor volume and weight of animals treated with quercetin were lower than those of control [ （ 15. 8 ± 10. 1 ） mm^3 vs. （52.3 ±26.5 mm^3; （44.8±10.4） mg vs. （91.3 ±31.4） mg] ,IP3 content was lower than that of control[ （ 15.9 ±2.8 ） pmol/mg protein vs （ 35.3 ± 6.6 ） pmol/mg protein ], Bax mRNA expression was not significantly different between the 2 groups [ RI which was the gray degree multiply area of bax / the gray degree multiply area of β-actin （0.64 ± 0. 12 ） vs. （0.56 ± 0. 15 ） ] , Bax mRNA expression was higher in group treated with quercetin than that of control [ （ 3. 16 ±0.95 ） vs. （ 1.37 ± 0.48 ） ]. Conclusions Quercetin can inhibit growth of transplanted hepatocellular carcinoma of nude mice by reducing IP3 production and bax protein expression.

关 键 词：癌 肝细胞 染料木黄酮 基因 BAX 

分 类 号：R735.7[医药卫生—肿瘤]