水稻抗性淀粉相关SSR标记的初步研究  

Preliminary Studies on Resistant Starch-linked SSR Marker in Rice

作　　者：牟方贵 闫宗武 冉瑞林 滕建勋 陈永波[1] 杨朝柱[1] 李明辉[3] 吴殿星[4] 

机构地区：[1]恩施自治州农业科学院,恩施445000 [2]湖北恩施市崔坝农技服务中心,恩施445003 [3]湖北省农业厅油料中心,武汉430071 [4]浙江大学核农所IAEA合作中心,杭州310029

出　　处：《分子植物育种》2008年第3期432-438,共7页Molecular Plant Breeding

基　　金：国家科技支撑计划项目(2007BAD73B03);国际原子能机构(CRP120609);浙江省攻关(0406/2004C12020-3/4);重点项目(2006C20010)

摘　　要：将高抗性淀粉(resistantstarch,RS)突变体RS111与中等和低直链淀粉含量水稻品种II-32B和宜香B杂交,构建F2群体,发现位于第8染色体上的RM72和RM547和位于第6染色体上的RM217和RM225与RS相关。在杂交组合II-32/RS111的F2群体中,RM72和RM547与抗性淀粉和直链淀粉含量关系密切,出现了低亲本、杂合及高亲本3种基因型,且不同基因型间RS和AAC达1%显差异;在杂交组合宜香B/RS111的F2群体中,按标记RM217和RM225基因型可区分低与中高RS含量,其中RM225杂合型和高亲本类型间RS含量达5%差异水平。Four sets of simple sequence repeat （SSR） primers, RM72 and RM547 on chromosome 8, and RM217 and RM225 on chromosome 6 were found to linked with resistant starch CRS） content in rice by using two of separated F2 generations derived from the crosses ofhigh-RS mutant RS111 （Oryza sativa indica） to cv II-32B with intermediate amylose content rice （Oryza sativa indica rice） and to Yixiang B （Oryza sativajaponica rice） with low amylase content, respectively. In the F2 generation of II-32B/RS111, both of SSR primers RM72 and RM547 were closely linked to the contents of RS and amylose, and the amounts of RS and amylose were significantly different at 1% level among three types of genotypes such as low-parent type, heterozygote, high-parent type. In the F2 population of Yixiang B/RS111, plants with low RS content could be identified with SSR primer RM217 and RM225, and the contents of RS between heterozygote and high-parent type were significantly different at 0.05 levels when identified by SSR marker RM225.

关 键 词：水稻 抗性淀粉 SSR标记 

分 类 号：S511[农业科学—作物学]