小麦抗叶锈基因Lr24、Lr35的STS、SCAR标记在近等基因系(NILs)上的特异性验证  

Verification of STS Molecular Markers for Leaf Rust Resistance Genes Lr24 and Lr35 in Near-isogenic Lines of Wheat cv.Thatcher

作　　者：刘莉 陈云芳[2] 刘华梁[3] 杨文香[3] 张汀[3] 刘大群[3] 

机构地区：[1]河北省植保植检站,石家庄050011 [2]苏州出入境检验检疫局,苏州215126 [3]河北农业大学植物保护学院植物病理系,河北省农作物病虫害生物防治工程技术研究中心,保定071001

出　　处：《分子植物育种》2008年第3期471-474,共4页Molecular Plant Breeding

基　　金：河北省教育厅基金项目;国家973前期项目(2005CCAO1600)

摘　　要：用一套分别含有不同抗叶锈基因的53个以Thatcher为遗传背景的近等基因系(near-isogeniclines,NILs)对已报道的分别与抗叶锈基因Lr24和Lr35连锁的STS、SCAR进行特异性验证。结果对于与Lr24连锁的STS标记,在53个NILs中只在TcLr24亲本中扩增出片段大小与报道相同的310bp的条带,在TcLr35中也扩增出了一条片段,但片段大小不同于310bp约为270bp。对于与Lr35连锁的SCAR标记,只在TcLr35亲本中扩增出片段大小为900bp的条带,与报道片段大小一致。验证结果表明与抗病基因Lr24和Lr35连锁的STS、SCAR分子标记在NILs中特异性都较好,进一步证明了这两个分子标记可方便地用于小麦抗叶锈基因Lr24、Lr35的分子标记辅助选择育种。Sequence tagged site （STS） markers for Lr24 and Sequence characterized amplified regions （SCAR） marker for Lr35 against Puccinia triticina had been used to screen a set of near-isogenic lines-（NILs） of wheat cv.- Thatcher containing in total 53 different Lr genes and their alleles. Polymerase chain reaction （PCR） analysis was carried out using STS and SCAR primers specific for the leaf rust resistance genes Lr24, Lr35. The amplification product of the STSLr35-Sr39 marker for the Lr35 resistance gene was detected only in cultivar of TcLr35, The sizes of the amplified marker fragments were 900 bp, same as the reported in fragment size. The amplification product of the STSLr24.J9 marker for the Lr24 resistance gene was detected one 310 bp fragment in cultivar of TcLr24, the fragment size was same the reported, at the same time, a approximately 270 bp fragment was specifically amplified in TcLr35. The result show the marker STSLr24-J9 and STSLr35-Sr-39was unique and specific, and can be used for molecular marker-assisted selection.

关 键 词：小麦叶锈 抗叶锈病基因 STS分子标记 SCAR分子标记 近等基因系 

分 类 号：S512.1[农业科学—作物学] S435.121.4