胃良恶性病变组织中PSCA和Oct-4的表达及意义  

Expression and significance of prostate stem cell antigen and Oct-4 in benign and malignant lesions of the stomach

作　　者：姚宏亮[1] 杨竹林[1] 李永国[1] 

机构地区：[1]中南大学湘雅二医院普外科,长沙410011

出　　处：《中南大学学报（医学版）》2008年第7期623-627,共5页Journal of Central South University ：Medical Science

摘　　要：目的:研究胃良恶性病变组织中PSCA和Oct-4的表达及其临床意义。方法:58例胃癌、20例癌旁组织、36例淋巴结转移灶及80例不同类型胃良性病变组织常规制作石蜡包埋切片,PSCA和Oct-4染色方法均为EnVision免疫组织化学法,比较其在不同病理类型中的表达情况。结果:胃癌病例PSCA,Oct-4阳性率明显高于癌旁组织及不同类型胃良性病例(P<0.05或P<0.01),PSCA和Oct-4阳性表达癌旁组织和良性病例均呈不同程度不典型增生;淋巴结转移灶与相应原发灶比较,两者阳性率无明显差异(P>0.05);浸润深度T1+T2、无淋巴结转移、淋巴结转移N1站及无远处转移病例PSCA和Oct-4表达阳性率明显低于浸润深度T3+T4、淋巴结转移、淋巴结转移N2+N3站及远处转移病例(P<0.05或P<0.01)。结论:PSCA和Oct-4表达与胃癌侵袭能力、淋巴结转移及远处器官转移有密切关系,提示PSCA,Oct-4表达可能是反映胃癌生物学行为和预后的重要标记物。Objective To determine on the expression of prostate stem cell antigen （ PCSA ） and Oct-4 and detect their clinicopathological significance in benign and malignant lesions of the stomach. Methods EnVision immunohistochemistry for assaying the expression of PSCA and Oct-4 was used in paraffin-embedded sections from specimens of primary foci （ n = 58 ） and metastatic foci of regional lymph nodes （ n = 36） of gastric cancer, peritumoral tissues （ n = 20） , and benign lesions of the stomach （n = 80）. Results The positive rates of PSCA and Oct-4 were significantly higher in gastric cancer than those in peritumoral tissues and benign lesions （ P 〈 0. 05 or P 〈 0.01 ）. The positive cases of PSCA and Oct-4 in peritumoral tissues and benign lesions showed mildto severe-atypical hyperplasia. No difference of the positive rate of PSCA and Oct-4 was found between the primary foci and the metastatic foci of gastric cancer （ P 〉 0.05 ）. The positive rates of PSCA and Oct-4 were significantly lower in infiltrating depth T1 - T2 , non-metastasis of lymph nodes, metastasis of lymph nodes N1 site, and non-metastasis of distant organs than those in infiltrating depth T3 - T4 , metastasis of lymph nodes, metastasis of lymph node N2 - N3 site, and metastasis of distant organs （ P 〈 0.05 or P 〈 0.01 ）. Conclusion The expressive levels of PSCA and Oct-4 might be related to the invasive potental, metastasis of lymph nodes, and distant organs,it sugguested the expressions levels of PSCA and Oct-4 might be important markers for reflecting the biological behaviors and prognosis of gastric cancer

关 键 词：胃肿瘤 胃炎 胃溃疡 胃息肉 前列腺干细胞抗原 OCT-4 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]