双波长薄层扫描法测定解热止痛散中对乙酰氨基酚和咖啡因的含量  

DETERMINATION OF THE CONTENT OF PARACETAMOLUM AND CAFFEINUM IN ASPIRINI PULVIS COMPOSITUS WITH THIN-LAYER CHROMATOGRAPHIC SCANNER

作　　者：梁勇[1] 李全民[2] 张兰芳[2] 

机构地区：[1]华南师范大学化学系,广州510631 [2]河南师范大学化学系,河南新乡453002

出　　处：《华南师范大学学报（自然科学版）》1997年第3期53-57,共5页Journal of South China Normal University(Natural Science Edition)

摘　　要：在Cs- 930薄层扫描仪上以紫外反射吸收方式 ,灵敏快速测定了解热止痛散中对乙酰氨基酚和咖啡因的含量 .扫描参数 :对乙酰氨基酚λs=2 4 8.5nm ,λR=34 0 .0nm ,咖啡因λs=2 73.0nm ,λR=34 0 .0nm ,散射参数Sx=3.采用双波长反射线齿型扫描 ,狭缝 1.2mm× 1.2mm ,所用薄层板为GF2 54,薄层厚度 0 .4mm ,展开剂为氯仿 :甲醇∶醋酸乙酯∶冰醋酸 =10∶2∶2∶0 .2 (体积比 ) ,平均回收率对乙酰氨基酚为 95.6% ,咖啡因为 10 8.4 %A simple sensitive thin-layer chromatographic method is described for the determination of paracetamolum and caffeinum.The content of paracetamolum and caffeinum were measured λ s=248.5nm,λ R=340nm,λ s=273nm,λ R=340nm, respectively with a SHIMADZU Cs-930 TLC scanner in absorption mode.The TLC plates used were GF254.The thickness of plates was 0.4mm.Trichloromethane-methanol-ethy1 acetate-glacial acetic acide (10∶2∶2∶02) was used in developed reagent.the average recovery of paracetamolum and caffeinum were 95.6% and 108 4% respectively.

关 键 词：对乙酰氨基酚 咖啡因 TCL 解热止痛散 薄层扫描 

分 类 号：R971.1[医药卫生—药品] R927.2[医药卫生—药学]