天蚕素A-蜂毒素杂合肽基因的克隆  

CLONING OF A GENE ENCODING CECROPIN A(1-7) MELITTIN (5-12) HYBRID

作　　者：梁山[1,2] 刘飞鹏[1,2] 

机构地区：[1]华南师范大学生物系 [2]暨南大学生物工程系

出　　处：《华南师范大学学报（自然科学版）》1997年第4期71-75,共5页Journal of South China Normal University(Natural Science Edition)

摘　　要：杂合肽天蚕素（１－７）蜂毒素（５－１２）是一个只有１５个氨基酸残基的短肽，是具有疏水性Ｃ－端和亲水性Ｎ－端的双性分子．它的分子大小只相当于天蚕素Ａ的４０％，而抗菌活性相当于完整天蚕素，或更高．本文根据其氨基酸顺序设计一个４５ｂｐ的杂合肽基因，通过接头克隆到载体ｐＶＴ１０２－Ｕ上，在酵母中表达，用比浊法初步测定表明表达产物具有抗Ｅ．ｃｏｌｉＤ３１活性．Cecropin A(1-7)Melittin(5-12)hybrid is a shortened 15-residue peptide which has a hydrophobic C-terminus and a hydrophilic N-terminus with only 40% size of cecropin A and antibacterial activity is similar to the whole one,or even higher.We design and synthesis a 45bp gene encoding this hybrid according to the aminoacid sequence.Then the gene is cloned to the express vector pVT102-U by linker and adapter and is expressed in yeast.The antib acterial activity of gene product is detected by spectraphotometry and is exhibited to inhibit E.coli D31.

关 键 词：天蚕素 蜂毒素 抗菌肽 基因克隆 酵母 表达载体 

分 类 号：Q966[生物学—昆虫学] Q78