高铁饲料对大鼠铁水平及铁调素mRNA表达的影响  

Effect of higher iron in diet on iron levels and hepcidin mRNA expression levels in rats

作　　者：韩巍[1] 王朝旭[1] 苏畅[1] 徐小磊[1] 史锐[1] 蓝岚[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生教研室,哈尔滨150081

出　　处：《卫生研究》2008年第4期474-476,共3页Journal of Hygiene Research

摘　　要：目的研究不同铁含量的高铁饲料对大鼠体重、体内铁水平及铁调素mRNA的影响。方法60只清洁级雄性SD大鼠随机分为6组,分别喂饲铁含量为35(A组)、100(B组)、200(C组)、500(D组)、1000(E组)、3500(F组)mg/kg的饲料8周后,处死大鼠。取大鼠肝脏、十二指肠和血清,比色法测定大鼠血清铁(SI)、转铁蛋白饱和度(TS)、ELISA法测定血清铁蛋白(SF)水平;逆转录-聚合酶链反应法检测大鼠肝脏铁调素(hepcidin)和十二指肠膜铁转运蛋白(ferroprotin,Fpn1)mRNA表达水平。结果随着饲料铁水平的提高,大鼠体重增重逐渐减少;大鼠血清铁、血清铁蛋白、转铁蛋白饱和度的水平、肝脏铁调素mRNA的表达水平逐渐升高;FPN1mRNA的表达下降。结论高铁饲料对大鼠体重、血清铁水平及肝脏铁调素、十二指肠Fpn1mRNA的表达均产生影响。Objective To study the effects of higher iron in diet on weight, iron status and hepcidin mRNA expression level in rats. Methods Sixty clean male SD rats were randomly divided into 6 groups according to the weight and were administrated higher iron at the doses of 35,100,200,500,1000,3500mg/kg of irons, respectively. Rats were killed after 8 weeks and serum and liver and duodenum of rats were collected. The serum iron levels, transferrin saturation were measured by colorimetric method. The serum ferrtin concentrations were measured by ELISA. The hepcidin mRNA level in liver and ferroportin mRNA expression in duodenum were measured by RT-PCR. Results After 8 week treatments, there was significant difference of the weights among the six groups. With the increases of iron levels in diet, the levels of serum iron, serum ferrtin, and transferrin saturation were gradually increased, and the hepcidin mRNA expression level in liver significantly increased, and the expression of ferroprotin mRNA expression level in duodenum was decreased. Conclusion Higher iron diet could influence the weights, serum iron status, liver hepcidin and duodenum ferroprotin mRNA expression levels of the rats.

关 键 词：铁 铁负荷 肝脏铁调素 铁转运蛋白 饲料 铁调素 

分 类 号：R151.3[医药卫生—营养与食品卫生学] Q581[医药卫生—公共卫生与预防医学]