HPLC法测定鳗鱼中三种喹诺酮药物残留  

Determination of Three Kinds of Quinolones in Eels by High Performance Liquid Chromatography

作　　者：张健玲[1] 张勇清[1] 黄慧贤[1] 林银婷 

机构地区：[1]江门出入境检验检疫局,广东江门529000

出　　处：《中国食品卫生杂志》2008年第4期316-318,共3页Chinese Journal of Food Hygiene

摘　　要：目的建立一种高效液相色谱方法测定鳗鱼中环丙沙星、恩诺沙星和恶喹酸3种喹诺酮类药物残留量，方法试样用含1％乙酸的乙腈提取，用正己烷脱脂、浓缩，缓冲盐＋乙腈（80＋20，体积分数）溶解，超高速离心净化，用荧光检测器测定，外标法峰面积定量。结果试样在10、0～60．0μg／kg范围内校正曲线呈良好的线性关系，3种物质的相关系数都在0．999以上。加标回收率：恶喹酸为87．4％～105．1％，环丙沙星为70．0％～85．7％，恩诺沙星为76.0％～92．8％，相对标准偏差均小于10％；检出限：恶喹酸为1.4μg／kg，环丙沙星为2．7μg／kg，恩诺沙星为1．5μg／kg。结论该方法操作简单、快捷．结果准确、可靠。Objective To determine three kinds of quinolones （ciprofloxacin, enrofloxacin and oxclinic acid ） residues in eels by high performance liquid chromatography （HPLC）. Method The samples were extracted with 1% acetic acid-acetonitrile, then defatted with hexane and concentrated by evaporation. The residues were dissolved in the buffer-acetonitrile. After high-speed centfifugation, the solutes were determinated by HPLC - FLD, and quantified by external standard method. Results The linear ranges of standard curves were 10.0 - 60.0 μg/kg, and the correlation coefficients were more than 0.999. The recoveries in spiked samples of Oxolinic acid, Ciprofloxacin hydrochloride and Enrofloxacin were 87.4 - 105.1%, 70.0 - 85.7% and 76.0 92.8%, respectively. The RSDs were all below 10%. The detection limits of Oxolinic acid, Ciprofloxacin hydrochloride and Enrofloxacin were 1.4 μg/kg, 2.7 μg/kg and 1.5 μg/kg, respectively. Conclusion The method could be simple, fast, accurate and credible.

关 键 词：喹诺酮类 环丙沙星 鳗 色谱法 高压液相 

分 类 号：R155.5[医药卫生—营养与食品卫生学]