Caspase 8和Caspase 3在TRAIL诱导神经母细胞瘤细胞凋亡中的作用(英文)  

Role of Caspase 8 and Caspase 3 in TRAIL-induced apoptosis of neuroblastoma cells

作　　者：佟海侠[1] 金冬岩[2] 张继红[1] 张锦华[1] 

机构地区：[1]中国医科大学附属盛京医院血液研究室,辽宁沈阳110022 [2]中国医科大学附属盛京医院检验科,辽宁沈阳110022

出　　处：《中国现代医学杂志》2008年第14期1953-1957,共5页China Journal of Modern Medicine

基　　金：the National Natural Science Foundation of China (No.39470739);the Research Foundation of Ministry of Public Health (No.20122167);Doctor Priming-Natural Science Foundation of Liaoning Province (No.20041047);the Governmental Technology Foundation of Liaoning Province( No.2005225013-5)

摘　　要：目的探讨 Caspase 8 和 Caspase 3 在肿瘤坏死因子相关凋亡诱导配体( TRAIL) 诱导 CHP212 神经母细胞瘤细胞凋亡中的作用。方法应用流式细胞仪检测 TRAIL、Caspase 8/Caspase 3 抑制剂 +TRAIL 对CHP212 细胞的诱导凋亡作用。应用比色法测定 Caspase 8、Caspase 3的相对活性。应用透射电镜对凋亡细胞进行形态学的观察。结果 TRAIL 可诱导 CHP212细胞的凋亡,并存在剂量依赖性;Caspase 8 /Caspase 3抑制剂能抑制TRAIL对CHP212细胞的诱导凋亡作用。随 TRAIL 作用时间的延长,Caspase 8、Caspase 3的活性逐步升高,分别于作用16h、8h后达高峰透射电镜可见到典型的细胞凋亡特征。结论TRAIL通过Caspase信号传导通路诱导CHP212细胞凋亡并伴随 Caspase 8和 Caspase 3活性的增高。[Objective] To study the role of Caspase 8 and Caspase 3 in TRAIL-induced apoptosis of neuroblastoma cell llne CHP212 cells. [Methods] The effects of TRAIL and Caspase 8/Caspase 3 inhibitor＋ TRAIL on the apoptosis of CHP212 cells were detected by flow eytometry. The relative Caspase 8/Caspase 3 activity was measured by eolorimetrie assay. The Morphology of the apoptosis cells were detected by using the transmission electron microscope （TEM）. {Results] TRAIL could induce apoptosis of CHP212 cells in a dose dependent way. Caspase 8/Caspase 3 inhibitor could diminish the apoptosis of CHP212 cells induced by TRAIL. The relative Caspase 8/Caspase 3 activity of CHP212 cells increased gradually with the prolongation of TRAIL action time and reached the peak at 16 h and 8 h respectively. Typical features of apoptosis were seen by TEM. [Conclusion] TRAIL could induce apoptosis of CHP212 eeUs through Caspase - transduced signal pathway with the increase of Caspase 8 and Caspase 3 activity.

关 键 词：神经母细胞瘤 凋亡 半胱-天冬氨酸蛋白酶 肿瘤坏死因子相关凋亡诱导配体 

分 类 号：R739.4[医药卫生—肿瘤]