缺氧诱导因子-1在肝内胆管癌中的表达及其与血管内皮生长因子和微血管密度的关系  

The Expression of HIF-1α in human cholangiocarcinomas (ChC) and its relation to VEGF and MVD

作　　者：吕品[1] 聂盛丹[1] 曹友德[1] 梁珊[1] 徐伟[1] 石泳中[1] 李灼日[1] 

机构地区：[1]湖南师范大学附属第一医院,湖南长沙410005

出　　处：《中国现代医学杂志》2008年第14期1979-1982,共4页China Journal of Modern Medicine

摘　　要：目的探讨肝内胆管癌组织中缺氧诱导因子-1(HIF-1α)的表达及其与VEGF的表达、微血管密度(MVD)及临床病理因素之间的关系。方法采用免疫组化方法检测56例胆管癌及20例癌旁正常肝内胆管中HIF-1α、VEGF的表达,用CD34单克隆抗体标记血管内皮细胞并计数MVD,分析其间的相关性及其与肿瘤临床病理特征之间的相关性。结果肝内胆管癌组织中HIF-1α和VEGF的阳性表达率显著高于正常组织(P<0.05);HIF-1α和VEGF的表达均与淋巴结转移和TNM分期呈正相关(P<0.05);HIF-1α的表达与VEGF的表达和MVD值呈正相关(P<0.05)。结论HIF-1α在胆管癌组织中明显上调,与肿瘤血管生成、肿瘤转移及预后密切相关;提示检测HIF-1α可能成为判断胆管癌生物学行为及预后的重要指标,并作为临床控制胆管癌的作用靶点。[Objeelive] To investigate the expression of HIF-1α gene and its relation to vascular endothelial growth faetor （VEGF） protein and mierovessei density （MVD） in human eholangioeareinomas （ChC）. [Methods] The expression of HIF-1α and VEGF were asessed by immunohistoehemieal staining in 56 eases with human eholangio- eareinomas （ChC） and in 20 eases with surrounded normal tissues, MVD was determined by anti-CD34 Endoglin im- munohitoehemieal staining and then we studied the relationship between the expression and the elinie opathologieal feature. [Results] The expressions of HIF-1α and VEGF in ChC were significantly higher than those in normal tissues （P 〈0.05）. The expression of HIF-1α and VEGF were both eorrelated with lymph node involvement （P 〈0.05） and elinleal stage （P 〈0.05）; the expression of HIF-1α was signifieanfly eorrelated with the VEGF expression and MVD （P 〈0.05）. [Conclusions] The upregnlation of the expression of HIF-1α in ChC had a close relationship to tumor angionesis, earnogenesis, development and infdtration. The overexpression of HIF-1α and VEGF can be used as important indiees for evaluatinge biologieal behaviors and prognosis of HIF-1α, and may beeome a new target for tumor treatment.

关 键 词：HIF-1 VEGF MVD 肝内胆管癌 免疫组织化学 

分 类 号：R735.8[医药卫生—肿瘤]