人乳头瘤病毒与食管鳞癌及不典型增生相关性的研究  

Study of association between HPV infection and squamous cell cancer as well as dysplasia tissues of esophageal

作　　者：刘铭[1] 张晓莉[2] 府伟灵[2] 杨军民[1] 朱静[2] 夏梅[1] 

机构地区：[1]第三军医大学西南医院胸外科,重庆400038 [2]第三军医大学西南医院基因诊断治疗中心,重庆400038

出　　处：《中国现代医学杂志》2008年第14期2084-2086,共3页China Journal of Modern Medicine

摘　　要：目的研究重庆地区食管癌组织中人类乳头状瘤病毒(HPV)感染与食管鳞癌发生的关系。方法收集112例食管鳞状细胞癌标本和38例非典型增生食管上皮及74例正常食管黏膜组织。运用荧光定量PCR方法分别检测各组织标本中HPV-16、18型的感染状况。结果食管鳞癌组织、非典型增生上皮及正常黏膜中HPV-16DNA的检出率分别为38.4%(43/112)、15.9%(6/38)和5.4%(4/74)。鳞癌组织中HPV-16的检出率明显高于非典型增生上皮和正常食管黏膜(P<0.05),非典型增生上皮中HPV-16的检出率虽远高于正常食管黏膜,但统计学差异未见显著性;食管鳞癌中HPV-16的阳性检出率与其分化程度未见明显相关。所有组织标本均未检出HPV-18DNA。结论HPV-16的感染可能是重庆地区食管鳞癌发生的之一高危因素;积极开展防治高危型HPV感染对降低该地区食管鳞癌的发病率具有重要的意义。[Objective] To investigate the association between human papillomavirus （HPV） infection and esophageal squamous cell cancer （ESCC）. [Methods] HPV-16, 18 DNA was examined in 112 ESCC specimens, 38 dysplasia tissues and 74 normal esophageal mucosa tissues, respectively by using fluorescence quantitative PCR. [Results] The detection frequencies of HPV-16 DNA in 112 ESCC, 38 dysplasia tissues and 74 normal mucosa tissues were 38.4% （43/112）, 15.9% （6/38） and 5.4% （4/74）, respectively. The detection frequency of HPV-16 was significantly higher in ESCC than in dysplasia tissues and normal ones. The detection frequency of HPV-16 was also higher in dysplasia tissues than in normal mucosa, while no statistical significance was found. No significant associ- ation was observed between the detection frequency of HPV-16 and the differentiation of ESCC. HPV-18 DNA was not detected in any of tissue specimens. [Conclusions] HPV-16 infection may be a high-risk factor for ESCC in Chongqing local area, and therefore the prevention and therapy of HPV infection would be of value for the reduction of incidence of ESCC in this aera.

关 键 词：食管鳞癌 人类乳头状瘤病毒 荧光定量PCK 

分 类 号：R735.1[医药卫生—肿瘤]