丁酸钠对人乳腺癌细胞株MCF-7细胞增殖及p27^(Kip1)蛋白表达影响的研究  

Effect of sodium butyrate on proliferation of MCF-7 and expression of p27^(Kip1) in human breast cancer cell lines

作　　者：谢瑞莲[1] 管晓翔[1] 陈龙邦[1] 王靖华[1] 耿怀成[1] 朱宝立[2] 

机构地区：[1]南京军区南京总医院肿瘤内科,江苏南京210002 [2]江苏省疾病预防控制中心,江苏南京210009

出　　处：《中华肿瘤防治杂志》2008年第14期1041-1044,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30470669);江苏省科技厅社会发展项目(3S2005661)

摘　　要：目的:通过观察组蛋白去乙酰化酶抑制剂(histon-deacetylase inhibitors,HDACIs)丁酸钠(sodium butyrate,NaB)对人乳腺癌细胞株MCF-7细胞的增殖影响以及p27Kip1蛋白表达改变,探讨NaB调控乳腺癌细胞增殖的分子机制。方法:乳腺癌MCF-7细胞经不同浓度NaB作用后,相差显微镜观察细胞形态变化和细胞增殖情况,流式细胞仪分析细胞周期分布,免疫组化检测p27Kip1蛋白表达。结果:NaB对MCF-7细胞有显著的增殖抑制作用,呈时间剂量依赖性,处理后的MCF-7细胞出现凋亡形态变化;细胞周期阻滞于G0/G1期,NaB 2mmol/L组G0/G1期达(62.2±2.2)%,4mmol/L组G0/G1期达(78.1±3.8)%,空白组G0/G1期达(53.1±2.4)%,P<0.05;p27蛋白表达水平上调。结论:NaB可抑制乳腺癌细胞的生长,该作用可能与p27蛋白表达增加有关。OBJECTIVE： To investigate the effect of sodium butyrate （NaB）on proliferation of human breast cancer cell MCF-7 and expression of p27^kip1. METHODS： A human breast cancer cell line MCF-7 was treated with different concentrations of NaB. A light microscope was used to find out morphological changes in carcinoma cells. The cell cycle status were investigated by flow cytometry, The expression of p27^kip1 was determined by the immunocytochemical assay. RESULTS： NaB significantly inhibited the proliferation of MCF-7 human breast cancer cells in a time- and dosedependent manner. MCF-7 cells treated with NaB was arrested in G0,/G1 phase,（62.2±2.2）% in 2 mmol/L NaB group, （78.1±3.8）% in 4 mmol/L NaB group, and （53.1±2.4）% in control group. Compared with the blank control group, NaB groups were significantly increased, P〈0.05. The expression of p27^kip1 was markedly enhanced after being treated with NaB. CONCLUSION.. NaB can inhibit the growth of breast cancer cell in vitro, which might be associated with the change of p27^kip1 protein expression.

关 键 词：丁酸类/药理学 乳腺肿瘤/病理学 细胞周期 P27蛋白 

分 类 号：R737.9[医药卫生—肿瘤]