乳腺癌患者骨髓微转移与其他预后指标相关性的研究  

Correlation between bone marrow micrometastasis and conventional prognostic factors in patients with breast cancer

作　　者：张丽丽[1] 顾禾[1] 时昌文[1] 曹莉莉[1] 孙靖中[2] 

机构地区：[1]山东大学附属千佛山医院普外中心乳腺甲状腺外科,山东济南250014 [2]2山东大学齐鲁医院乳腺外科,山东济南250012

出　　处：《中华肿瘤防治杂志》2008年第14期1076-1079,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:研究乳腺癌患者骨髓微转移与其他预后指标的相关性。方法:采用RT-PCR法检测了69例乳腺癌患者骨髓中CK19 mRNA的表达,并将骨髓微转移与肿瘤细胞DNA含量、细胞周期分布及增殖细胞核抗原(PCNA)、血管内皮细胞生长因子(VEGF)、HER-2和ER等指标和传统预后指标的相关性进行了研究。结果:RT-PCR技术检测乳腺癌患者骨髓微转移的阳性率为34.8%。骨髓微转移与肿瘤大小、患者年龄、月经状态及激素受体状况等指标无相关性,P>0.05。骨髓微转移与腋窝淋巴结状态、HER-2过表达、VEGF水平、S期细胞比值(SPF)以及PCNA有相关性,P<0.05。在淋巴结病理学检查阴性的病例中,有17.6%的病例骨髓微转移检查阳性。结论:乳腺癌患者骨髓微转移与患者的一般临床特点无相关性,但与反映肿瘤细胞增殖、侵袭特性的大部分生物学指标具有相关性。在淋巴结病理学检查阴性的病例中,部分病例骨髓微转移阳性。OBJECTIVE： To study the correlation between bone marrow micrometastasis and other conventional prognostic factors in patients with breast cancer. METHODS： The expression of CK19 mRNA in bone marrow from 69 breast cancer patients was detected with RT-PCR. The content of DNA, the distribution of cell cycle and the value of PCNA were investigated by flow cytometry. VEGF was tested with ELISA and the expressions of HER-2 and ER were detec- ted by immunohistochemistry. The correlation of bone marrow micrometastasis with the content of DNA, DNA index, SPF, PCNA and traditional prognostic factors was studied. RESULTS： The positivity of bone marrow micrometastasis tested by RT-PCR in breast cancer patients was 34.8%. There was no correlation between bone marrow micrometastasis and tumor size, or patient＇s age, or menstrual status or ER, P〉0.05. However, bone marrow micrometastasis was correlated with lymph node involvement, VEGF level, the over-expression of HER-2 gene and other proliferating status including DNA index, S phase fraction and PCNA, P〈0.05. In node-negative patients, the positivity of bone marrow micrometastasis was 17. 6%. CONCLUSIONS： Bone marrow micrometastasis has no correlation with the common clinical features while does have correlation with those biological factors indicating cell proliferation or invasion. Some of the patients with tumor-negative ALNs have the positive bone marrow micrometastasis.

关 键 词：乳腺肿瘤 肿瘤转移 逆转录聚合酶链反应 预后 

分 类 号：R737.9[医药卫生—肿瘤]