人子宫内膜癌细胞株HECCL-1生物学特性的研究  

Further observation on biological characteristics of HECCL-1 cell line of human endometrial carcinoma

作　　者：徐云[1] 盛修贵[2] 张小玲[2] 王兴武[3] 宋现让[3] 魏玲[3] 

机构地区：[1]首都医科大学附属北京天坛医院妇产科,北京100050 [2]山东省肿瘤医院妇瘤科,山东济南250117 [3]山东省肿瘤医院基础研究中心,山东济南250117

出　　处：《中华肿瘤防治杂志》2008年第11期817-821,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：山东省自然科学基金(Q98C02125)

摘　　要：目的：探讨HECCL-1细胞株的生物学特性。方法：利用流式细胞仪测定耐药相关基因蛋白MDR1、GST—P、LRP、MMT和MRP及凋亡相关基因Fas、p53、Bcl-2和p16的表达，检测细胞DNA周期和瘤细胞接种裸鼠后成瘤情况；采用MTT法测定HECCL～1细胞在不同剂量6MVX线照射后的存活分数，计算放射生物学参数，并和HeLa细胞株作比较，计算照射前后细胞倍增时间。结果：HECCL-1细胞耐药基因及相关蛋白、凋亡相关基因均呈阴性表达。DNA周期检测结果：G0～G1期88．63％，G2～M期6．74％，S期4．63％。瘤细胞接种裸鼠后可再次成瘤并有肝转移表现；HECCL-1细胞株对射线敏感，肿瘤细胞群体倍增时间照射前后差异有统计学意义，P〈0．01。结论：HECCL-1细胞株耐药基因、凋亡基因、细胞DNA周期的检测及放射敏感性的研究结果，有助于指导子宫内膜癌的治疗。OBJECTIVE： To explore the biological characteristics of human endometrial carcinoma cell line-1 （HECCL-1）. METHODS： The drug resistance gene, apoptosis relative gene and cell DNA cycle were detected by the flow cytometry （FCM）. The surviving fraction after irradiation using 6MV X-ray at the different doses was determined by MTT assay. The radiobiological indices were calculated and campared to HeLa cell line. RESULTS： The expressions of drug resistance gene and apoptosis relative gene were all negative. The cell cycle at G0/G1 phase was 88. 63%, 6.74% at G2/M phase, and 4.63% at S phase. HECCL-1 cells could form tumor and metastasize to the liver in nude mice. HECCL-1 cell line was radiosensitive, and the population doubling time was significant different between pre-and post-irradiation, P〈0. 01. CONCLUSION： The results of the present study on HECCL-1 cell line are helpful in the treatment of human endometrial carcinoma.

关 键 词：子宫内膜肿瘤/生物学 放射生物学 基因表达 放射敏感性 

分 类 号：R737.33[医药卫生—肿瘤]