胰腺癌胰液蛋白质组学分析  

Comparative proteomics analysis on pancreatic cancer juice

作　　者：田美[1] 崔亚洲[1] 宗美娟[1] 陈彧[1] 韩金祥[1] 

机构地区：[1]山东省医学科学院山东省医药生物技术研究中心国家卫生部生物技术药物重点实验室山东省医学分子生物学重点实验室,山东济南250062

出　　处：《中华肿瘤防治杂志》2008年第15期1140-1143,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：山东省科技厅重点攻关资助项目(2005GG1102003)

摘　　要：目的:利用比较蛋白质组学方法鉴定胰腺癌早期诊断的蛋白标志。方法:采用双向电泳(2-DE)分离比较9个胰腺导管腺癌患者和9个非胰腺癌患者的胰液蛋白质表达谱,用肽质量指纹图谱(PMF)为基础的基质辅助激光解析离子化飞行时间串联质谱(MALDF-TO-F-MS/MS)对差异蛋白进行鉴定。采用蛋白质印迹方法进一步验证蛋白质MMP-9和DJ-1在胰液中的表达。结果:2-DE显示共24个蛋白质点出现>2.0倍的表达差异,上调14个,下调10个。蛋白质印迹检测显示在胰腺癌胰液中高表达MMP-9和DJ-1蛋白。结论:MMP-9和DJ-1可能成为通过内镜下逆行胰胆管造影术(ERCP)筛查胰腺癌高危患者及早期诊断胰腺癌的潜在的胰液肿瘤标志。OBJECTIVE： To identify protein markers for the early diagnosis of pancreatic cancer by a comparative proteomic method. METHODS： A comparative analysis on the pancreatic juice protein profiling from 9 pancreatic cancer patients and 9 cancer-free controls was carried out by two-dimensional gel eleetrophoresis （2-DE）, and differentially expressed proteins were identified by peptide mass fingerprint （PMF） based on matrix assisted laser desorption/ionization- time of flight tandem mass spectrornetry（MALDI-TOF MS/MS）. Matrix metalloproteinase-9（MMP-9）and Oncogene DJ1 （DJ-1）in pancreatic juice were further confirmed using western blotting. RESULTS： 2-DE displayed a total 24 protein spots with at least 2.0 fold of differential expression, of which 14 protein spots were up expressed and 10 were down-expressed in pancreatic cancer juices. Western blotting detection demonstrated MMP 9 and DJ-1 highly expressed in pancreatic juice from pancreatic cancer compared with cancer-free controls. CONCLUSION： MMP 9 and DJ-1 proteins might be promising biomarker candidates for screening of high-risk patients undergoing ERCP and the early diagnosis of pancreatic cancer.

关 键 词：胰腺肿瘤 胰液 蛋白质组学 肿瘤标志 生物学 

分 类 号：R735.9[医药卫生—肿瘤]