卵巢癌组织与患者血清中肽基精氨酸脱亚氨酶4和瓜氨酸化抗凝血酶表达的初步研究  

Expressions of PADI4 and citrullinated antithrombin in tissues and sera of patients with ovary carcinoma

作　　者：常建芳[1] 常晓天[1] 赵燕[1] 王志宇[1] 孟祥爱 陈锡良[2] 

机构地区：[1]山东省医药生物技术研究中心,山东济南250062 [2]章丘市人民医院检验科,山东章丘250200

出　　处：《中华肿瘤防治杂志》2008年第15期1169-1172,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:检测卵巢癌组织和患者血清中肽基精氨酸脱亚氨酶4(PADI4)和瓜氨酸化抗凝血酶(citrullinated antithrombin,cAT)的表达情况。方法:RT-PCR和实时荧光定量PCR检测11例卵巢癌组织PADI4mRNA表达;ELISA方法检测29例卵巢癌患者、11例卵巢癌手术后患者和23名正常人血清中PADI4和cAT表达。结果:PADI4mRNA在卵巢癌组织中的阳性表达率82%,表达量的中位数为1.57×102copies/mL;卵巢癌组(0.5053±0.0477)和手术后组(0.3941±0.0351)患者血清中PADI4表达水平高于正常对照组(0.1488±0.0148)的表达,P<0.05;卵巢癌组(0.442±0.077)和手术后组(0.3742±0.0833)患者血清中cAT表达水平高于正常对照组(0.2156±0.0116)的表达,P<0.05;卵巢癌组PADI4与cAT表达水平呈明显相关,P<0.01。结论:PADI4和cAT在卵巢癌中表达水平升高且两者密切相关,提示PADI4可能通过使抗凝血酶瓜氨酸化而参与了卵巢癌的发生发展过程。OBJECTIVE： To investigate the expressions of PADI4 and cAT in ovary carcinoma, METHODS： The expression of PADI4 transcripts in ovary carcinoma tissues （n=11 ） was measured by RT PCR and Taqman real time quantitative PCR. Expressional levels of PADI4 and cAT in sera of patients with o vary carcinoma （n= 29）, sera of the patients after excision sur gery （n =11） and sera of the health volunteers （n=23） were measured by ELISA and Sandwich ELISA with absorbance at 405 nm. RESULTS： PADI4 mRNA was detected in 82 % of the ovary carcinoma and the mean level of the expression was at 1.57 × 10^2 copies/mL in the samples. The mean levels of PADI4 in ovary carcinoma group, after surgery group and control groups were 0. 505 3±0. 047 7, 0. 3941 ±0. 035 1 and 0. 148 8±0. 014 8, respectively. The mean levels of cAT in the 3 group were 0. 442±0.077, 0.374 2±0.083 3 and 0.215 6±0.011 6 in that order. Statistics analysis revealed a positive correlation between the ex pressional levels of PADI4 and cAT in ovary carcinoma group （P〈0.01）. CONCLUSION： PADI4 and cAT can increase expressions in blood of patients with ovary carcinoma, which support PADI4 may be involved in carcinogenesis and development of the tumor by citrullination of antithrombin.

关 键 词：卵巢肿瘤 肽基精氨酸脱亚氨酶4 抗凝血酶 瓜氨酸化 

分 类 号：R737.31[医药卫生—肿瘤]