Hydrolysate of corn starch saccharified with a dual-enzyme system and the effect on the crystallization of itaconic acid when used in fermentation  

作　　者：李丕武 Chen Xiguang Liu Jianjun Wang Lingchong 

机构地区：[1]College of Marine Life Science, Ocean University of China, Qingdao 266003, P.R. China [2]The Key Lab of Food and Fermentation of Shandong, Jinan 250013, P.R. China

出　　处：《High Technology Letters》2008年第3期316-320,共5页高技术通讯（英文版）

基　　金：the National Natural Science Foundatioo of China(No.30670566);the International S&T Cooperation Program of China(No.2006DFA33150)

摘　　要：An improved process of hydrolysis of corn starch was adopted in the production of itaconic acid （IA）, the aim was to decrease the unfermentable reducing sugar （RS） in the medium from the beginning of the fermentation and to increase the crystallization efficiency of IA from the fermentation broth. The glucose （GS） syrups saccharified by several combinations of glucoamylase and pullulanase were investigated and used as the carbohydrate source of the fermentation medium for the spore-initiated submerged fermentations experiments. Compared with the conventional process （with pullulanase controlled）, the improved process decreased th.e RS residue in the fermentation broth from 3.01g/L to 1.35g/L and from 4.25g/L to 3.25g/L when the original RS of the medium were 100 and 120g/L, respectively. The crystallization efficiency of IA increased from 65% to 78.8% and from 69.58% to 82.81% with the original RS being 100 and 120g/L, respectively.An improved process of hydrolysis of corn starch was adopted in the production of itaconic acid(IA),the aim was to decrease the unfermentable reducing sugar (RS) in the medium from the beginningof the fermentation and to increase the crystallization efficiency of IA from the fermentation broth.Theglucose (GS) syrups saccharified by several combinations of glucoamylase and pullulanase were investi-gated and used as the carbohydrate source of the fermentation medium for the spore-initiated submergedfermentations experiments.Compared with the conventional process (with ptdlulanase controlled),theimproved process decreased the RS residue in the fermentation broth from 3.01g/L to 1.35g/L and from4.25g/L to 3.25g/L when the original RS of the medium were 100 and 120g/L,respectively.The crys-tallization efficiency of IA increased from 65 % to 78.8 % and from 69.58 % to 82.81% with the originalRS being 100 and 120g/L,respectively.

关 键 词：itaconic acid PULLULANASE SACCHARIFICATION 

分 类 号：Q814[生物学—生物工程]