CR2在实验性大鼠脑梗死后继发性脑损伤中的作用  

The role of CR2 in secondary brain injury after MCAO in rats

作　　者：张祥建[1] 祝春华[1] 刘春燕[1] 李海燕[1] 张丽英[1] 胡书超[1] 刘瑞春[1] 李春岩[1] 

机构地区：[1]河北医科大学第二医院神经内科,石家庄050000

出　　处：《脑与神经疾病杂志》2008年第4期428-431,共4页Journal of Brain and Nervous Diseases

基　　金：河北省重大科技攻关项目(06276103d)

摘　　要：目的:探讨补体受体2型(Complement Receptortype2,CR2)在实验性脑梗死(cerebral infarction,CI)后继发性脑损伤中的作用。方法:应用实验性大鼠大脑中动脉梗死模型(middlecerebral artery occlusion,MCAO),于术后6h、24h、48h、72h和7d采用Longa评分法、肢体对称试验评分法、Berderson评分法和平衡木评分法等4种方法进行行为学评分;用免疫组化染色观察炎性细胞浸润;用单克隆CR2抗体检测病灶周围脑组织CR2表达。结果:大鼠脑梗死后脑水肿、炎性细胞浸润均于48h达到高峰,CR2的表达高峰在术后6h即有表达,24h～72h达高峰,至7d仍有表达,CR2表达与脑梗死后脑水肿及脑损伤存在时间上相关,且与对照组相比有统计学意义,P<0.05。结论:CR2在脑梗死后脑组织损伤和脑水肿的形成中起到了一定的作用。Objective：To investigate the Complement Receptor type 2（CR2） in the brain injury after the model of middle cerebral artery occlusion（MCAO） in rats.Methods：60 male SD rats were subjected to right MCAO by insertion of an intraluminal nylon suture.The behavior changes were observed by using four different methods,HE staining,the morphology,the TTC stain of brain tissue and CR2 immunohistochemistory staining were measured at 6 h,24 h,48 h,72 h and 7 d after operation.Results：Inflammatory cells especially neutrophils infiltrating peaked at 48 h,and the expression of CR2 in the rat brain peaked at 24 h～48 h too.The CR2 expression had correlation with secondary brain injury.after MCAO.Conclusion：CR2 may play some role in the developing of the secondary neuronal injury after CI in rats.

关 键 词：脑梗死 补体受体2型 

分 类 号：R743.34[医药卫生—神经病学与精神病学]