阿司匹林赖氨酸盐在体外对人乳腺癌细胞的抑制作用及其机制  

The inhibitory effect of aspisol on human breast cancer cells in vitro and its mechanism

作　　者：张月林[1] 叶云[1] 李筱俊[1] 李子广[1] 祝晓光[1] 

机构地区：[1]安徽蚌埠医学院药理教研室,蚌埠233030

出　　处：《肿瘤》2008年第7期563-566,共4页Tumor

基　　金：安徽省教育厅自然科学重点研究计划资助项目(编号:2005KJ047ZD)

摘　　要：目的:探讨非选择性环氧合酶-2(cyclooxygenese-2,COX-2)抑制剂阿司匹林赖氨酸盐对乳腺癌MDA-MB-231细胞株COX-2、基质金属蛋白酶-9(matrix metalloproteinaseses-9,MMP-9)蛋白表达的影响。方法:不同浓度的阿司匹林赖氨酸盐作用于MDA-MB-231细胞24h后,HE染色观察细胞形态学的变化;采用MTT法检测阿司匹林赖氨酸盐对MDA-MB-231乳腺癌细胞增殖的抑制作用;应用Western印迹法分别检测阿司匹林赖氨酸盐对人乳腺癌MDA-MB-231细胞株COX-2、MMP-9蛋白表达的影响。结果:光学显微镜下阿司匹林赖氨酸盐处理组的细胞密度减小,细胞变圆,胞核染色变浅。阿司匹林赖氨酸盐对MDA-MB-231乳腺癌细胞的增殖有明显抑制作用(P<0.01)。与对照组相比,阿司匹林赖氨酸盐可显著抑制MDA-MB-231乳腺癌细胞COX-2、MMP-9蛋白的表达,并呈剂量-依赖效应(P<0.05)。结论:阿司匹林赖氨酸盐可抑制MDA-MB-231乳腺癌细胞COX-2、MMP-9蛋白的表达。Objective： To investigate the effects of aspisol on expressions of cyclooxygenese-2 （COX-2）, matrix metalloproteinaseses-9 （MMP-9） proteins in human breast cancer MDA-MB-231 cells. Methods： MDA-MB-231 cells were incubated with aspisol at various concentrations for 24 h. The morphological changes of MDA-MB-231 cells were evaluated by HE staining. The inhibitory effects of aspisol on the proliferation of MDA-MB-231 cells were determined by MTT assay. The effects of aspisol on the expressions of COX-2 and MMP-9 in MDA-MB-231 cells were measured by Western blotting. Results： Under light microscope the cells in aspisol treatment groups showed decreased density, round shape, and light staining of nucleus. Aspisol significantly inhibited the proliferation of MDA- MB-231 cells （P 〈0.01 ）. Compared with the control group, aspisol dose-dependently inhibited the expressions of COX-2 and MMP-9 protein in MDA-MB-231 cells （P 〈 0.05 ）. Conclusion： This study suggested that aspisol inhibited the expressions of COX-2 and MMP-9 protein in breast cancer MDA-MB-231 cells.

关 键 词：乳腺肿瘤 环氧合酶2抑制剂 基质金属蛋白酶类 基因表达 

分 类 号：R737.9[医药卫生—肿瘤] R730.53[医药卫生—临床医学]