Bcl-2家族成员在紫杉醇诱导胃腺癌细胞凋亡中的表达  

Expression of Bcl-2 Family Members in Paclitaxel-induced Apoptosis of Gastric Adenocarcinoma Cells

作　　者：沈茜[1] 苏浩[2] 张林杰[1] 

机构地区：[1]安徽医科大学免疫学教研室,合肥230032 [2]安徽医科大学附属省立医院,合肥230001

出　　处：《中国现代医生》2008年第21期162-163,172,共3页China Modern Doctor

基　　金：国家自然科学基金项目(编号:30572118);安徽省自然科学基金项目(编号:070413077)

摘　　要：目的研究Bcl-2家族成员在紫杉醇诱导胃腺癌细胞凋亡中的表达,探索紫杉醇作用的分子机制。方法流式细胞仪PI单染法检测细胞凋亡率;Western blotting检测紫杉醇诱导胃腺癌细胞凋亡过程中Bcl-2家族成员的表达水平变化。结果紫杉醇诱导胃腺癌细胞凋亡具有剂量和时间依赖性,SGC-7901较BGC-823胃腺癌细胞株对紫杉醇更为敏感。紫杉醇诱导胃腺癌细胞凋亡中,Bcl-2家族成员中促凋亡分子Bim表达水平增高,而抗凋亡分子Bcl-2、Bcl-xL表达降低。结论Bcl-2家族成员在紫杉醇诱导胃腺癌细胞凋亡中发挥重要的调节作用。Objective To study the expression of Bcl-2 family members in paclitaxel-induced apoptosis of gastric adenocarcinoma cells. Methods Apoptosis was measured with flow cytometry using propidium iodide staining. Western blotting was used to detect the target gene expression at protein levels. Results Paclitaxel induced apoptosis of gastric adenocarcinoma cells in a dose-and time-dependent manner. It appeared that SGC-7901 cells were more sensitive than BGC-823 cells to paclitaxel-induced apoptosis. Up-regulated Bim expression and down-regulated Bcl-2 and Bcl-xL in paclitaxel-induced apoptosis of gastric adenocarcinoma cells. Conclusion Bcl-2 family members play an important regulatory role in paclitaxel-induced apoptosis of gastric adenocarcinoma cells.

关 键 词：胃肿瘤 细胞凋亡 紫杉醇 BCL-2 

分 类 号：R735.2[医药卫生—肿瘤]