雌激素对青春期大鼠Sertoli细胞及血睾屏障的影响  

Estrogens Affect Sertoli Cells and the Blood-Testis Barrier in Pubertal Rats

作　　者：潘艺青[1] 郭强苏[1] 王如瑶[1] 徐晨[1] 

机构地区：[1]上海交通大学医学院组织学与胚胎学教研室

出　　处：《中华男科学杂志》2008年第7期590-596,共7页National Journal of Andrology

摘　　要：目的:研究在Sertoli细胞分化和成熟阶段,外源性雌激素对Sertoli细胞FasL表达以及血睾屏障的影响,进一步讨论影响血睾屏障的相关因素及其可能机制。方法:体内体外分别给与青春期SD大鼠大剂量的雌激素(二乙烯雌酚和雌二醇),运用免疫荧光方法和Western印迹观察Sertoli细胞内FasL的表达以及电镜下血睾屏障结构的变化。结果:外源性大剂量雌激素作用下,Sertoli细胞内FasL表达量明显增强(P<0.05);同时出现Sertoli细胞膜和生精上皮血睾屏障部位FasL的表达量上升;在18d实验组,电镜显示示踪剂镧透过血睾屏障,到达生精上皮全层。结论:外源性大剂量雌激素能使Sertoli细胞内FasL的表达量和分布区域发生改变并影响血睾屏障的结构。Objective：To investigate the correlation of exogenous estrogens with the expression of FasL in Sertoli cells and the blood-testis barrier during the differentiation and maturation period of Sertoli cells,and to discuss the related factors that influence the blood-testis barrier of pubertal rats.Methods：Super-physiological doses of exogenous estrogenic compounds（diethylstilbestrol and estradiol）were administered to pubertal Sprague-Dawley rats in vitro and in vivo,the FasL expression in the Sertoli cells of the rats detected by immunohistochemistry and Western blot,and the changes in the blood-testis barrier observed with the electron microscope.Results：After the exposure to exogenous estrogens,the FasL expression was markedly up-regulated in the immature Sertoli cells（P〈0.05）as well as in the Sertoli cell membrane and the blood-testis barrier of the epithelium.The tracer lanthanum passed through the blood-testis barrier and reached the whole layer of the epithelium at 18 days.Conclusion：Super-physiological dose of exogenous estrogens can change the expression and distribution of FasL in immature Sertoli cells and affect the structure of the blood-testis barrier.

关 键 词：雌激素 SERTOLI细胞 FASL 血睾屏障 大鼠 

分 类 号：R363[医药卫生—病理学]