生姜水提物对血管性痴呆模型大鼠的影响  

Effect of Aqueous Extract of Ginger on Vascular Dementia in Rats

作　　者：王军[1] 黄启福[2] 贾士奇[1] 王子华[1] 

机构地区：[1]河南省中医药研究院中药药理实验室,郑州450004 [2]北京中医药大学基础医学院

出　　处：《医学研究杂志》2008年第8期33-37,共5页Journal of Medical Research

基　　金：河南省属科研单位社会公益项目预研专项资金(0641130503)

摘　　要：目的探讨生姜水提物对血管性痴呆(VD)的治疗作用与机制。方法SD雄性大鼠随机分为假手术组,模型对照组,尼莫地平组,生姜水提物高、中、低剂量组。采用反复结扎与开放双侧颈总动脉的方法复制VD模型,Morris水迷宫定位航行实验和空间探索实验测定大鼠空间学习记忆能力,光化学比色法测定大脑皮质和纹状体乙酰胆碱酯酶(AChE)、SOD活性和MDA含量。结果与假手术组比较,VD对照组大鼠morris空间学习记忆能力和纹状体AChE活性明显降低,皮质AChE活性无明显变化;脑组织SOD活性和MDA含量分别有降低和升高的趋势,但无统计学意义。生姜水提物能明显提高VD大鼠morris定位航行能力和空间搜索能力;显著提高纹状体AChE活性,与模型对照组及石杉碱甲组比较均有显著性差异,对皮质AChE活性无明显影响;生姜高剂量能明显降低MDA含量,升高SOD/MDA比值。结论生姜具有提高血管性痴呆大鼠学习记忆能力的作用,作用机制与提高胆碱能神经功能和抑制胆碱酯酶活性有关。Objective To observe the therapeutic effects of aqueous extract of ginger on vascular dementia（VD） in rats. Methods Male SD rats were randomly divided into sham-operation group, model contral group, huperzine A group and three ginger aqueous extract groups for high, middle and low dosage. The VD rat model was established by bilateral corotid ligation and repeated cerebral ischemia reperfusion. Spatial learning and memory abilities in both place navigation test and spatial prob test were evaluated with Morris water maze. The activitie of acetylcholinesterase （ACHE） in cortex and striatum tissue and SOD and MDA content in brain tissue was detected by spectrophotometry. Results Compared with sham-operation group, the rats in model control group exhibited serious learning and memory deficits in both place navigation test and spatial prob test and decreased significantly AChE activity in striatum tissue. Aqueous extract of ginger could not only improve the learning and memory abilities in both place navigation test and spatial prob test, but also in-crease AChE activity and the ratio of SOD/MDA and decrease MDA content in brain tissue. Conclusion Aqueous extract of ginger has obvious effects on improving learning and momery abilities of rats with VD. Increasing the function of cholinergic neuron and inhibiting AChE activity are likely to be the partial mechanism.

关 键 词：电针 血管性痴呆 Morris迷宫 乙酰胆碱酯酶 大鼠 

分 类 号：R749.1[医药卫生—神经病学与精神病学] R338.26[医药卫生—临床医学]