DNA：23-Oxa-OSW-1生物靶标？  

DNA: A Biological Target of 23-Oxa-OSW-1?

作　　者：关文[1] 刘辉[1] 申杰[2] 汤平平[2] 吴厚铭[2] 张国安[1] 曹春阳[2] 

机构地区：[1]华中农业大学植物科技学院,武汉430070 [2]中国科学院上海有机化学研究所,上海200032

出　　处：《化学学报》2008年第14期1745-1748,共4页Acta Chimica Sinica

摘　　要：虎眼万年青皂苷OSW-1来源于百合科植物虎眼万年青,是一种具有良好抗肿瘤活性的皂苷成分.其类似物23-Oxa-OSW-1具有OSW-1一样潜在的活性,对恶性肿瘤细胞具有很强的抑制作用,IC50为0.052～0.4μmol·L-1.但OSW-1的生物靶标还没有报道.利用一维和二维1H NMR技术首次发现23-Oxa-OSW-1与DNA间有很强的相互作用.NMR数据分析表明23-Oxa-OSW-1中木糖部分可能参与了与DNA的结合.The anticancer drug, steroidal saponin OSW-1 was isolated from the bulbs of Ornithogalum saundersiae. Its analogue 23-Oxa-OSW-1 was found to strongly suppress the growth of the malignant tumor cells with an IC50 0.052-0.4 umol·L^-1, as potent as OSW-1. However its target in vivo is still unknown. In this paper, by using 1D and 2D lH NMR techniques, it was found that 23-Oxa-OSW-1 could interact with DNA. Further analysis of the NMR data indicated that its xylose part might be involved in its interaction with DNA. In addition, to our knowledge, the detailed assignment of 23-Oxa-OSW-1 1H chemical shifts done here has never been reported so far.

关 键 词：23-Oxa—OSW-1 NMR DNA 木糖 

分 类 号：R284[医药卫生—中药学]