紫外分光光度法测定盐酸奈福泮-β-环糊精包合物的含量  

Determination of nefopam hydrochloride(NFH) in nefopam NFH-β-cyclodextrin inclusion compound by UV spectrophotometry

作　　者：关皓月[1] 张纯[1] 顾伟锋[1] 

机构地区：[1]第二军医大学长征医院药学部,上海200003

出　　处：《药学实践杂志》2008年第4期291-293,共3页Journal of Pharmaceutical Practice

摘　　要：目的:建立测定盐酸奈福泮-β-环糊精包合物中盐酸奈福泮含量的方法。方法:采用紫外分光光度法测定含量,测定波长为267 nm。用乙酸乙酯洗脱未包封的盐酸奈福泮,测定被包封的盐酸奈福泮含量及包封率。结果:盐酸奈福泮检测浓度在0.01～0.4 mg/mL范围内线性关系良好(r=0.9995,n=9);日内RSD =0.17%(n=5),日间RSD=1.82%(n=5);平均加样回收率为100.39%(n=9);测得3批样品的包封率分别为73.28%、70.37%、74.97%。结论:该方法操作简便、结果准确,适用于盐酸奈福泮-β-环糊精包合物中盐酸奈福泮含量的测定。Objective：To establish a method for the content determination of NFH in NFH-β-cyclodextrin inclusion compound. Methods：The drug concentration in NFH-β-cyclodextrin inclusion compound was detected at the wavelength of 267 nm by UV spectrophotometry. The free NFH was eluated by cetoacetate. The enveloped NFH and its entrapment efficiency were detected by UV speetrophotometry. Results： The linear range of NFH was 0.01 - 0.4 mg/mL （ r = 0. 999 5, n = 9 ） ; the within day RSD and between-day were 0.17% and 1.82% （ n = 5） , respectively. The average recovery was 100.39% （ n = 9）. The entrapment efficiencies of 3 batches of samples were 73.28% ,70.37% ,74.97%. Conclusion：The method is simple and reliable,which can be used for the content determination of NFH in NFH-β-cyclodextrin inclusion compound.

关 键 词：紫外分光光度法 盐酸奈福-β-环糊精包合物 盐酸奈福泮 含量测定 

分 类 号：R927.2[医药卫生—药学]