晚期糖基化终产物对健康成人外周血单核细胞肿瘤坏死因子α和血红素加氧酶-1表达的影响  

Effects of advanced glycation end-products on expressions of TNF-α and HO-1 in peripheral monocytes in healthy adults

作　　者：卓凤婷[1] 薛耀明[1] 关美萍[1] 沙建平[1] 魏民[1] 邹艺[1] 曾展军[1] 

机构地区：[1]南方医科大学南方医院内分泌科,广州市510515

出　　处：《实用医学杂志》2008年第14期2375-2377,共3页The Journal of Practical Medicine

摘　　要：目的:探讨不同浓度晚期糖基化终产物(advanced glycation end-product,AGE)对健康成人外周血单核细胞肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)及血红素加氧酶-1(heme oxygenase-1,HO-1)表达的影响。方法:分离健康成人外周血单核细胞,随机分组,利用不同浓度体外制备的AGE修饰牛血清白蛋白(AGE-BSA)刺激,观察不同时间点每组细胞培养上清液中TNF-α表达量及细胞内HO-1mRNA的表达。结果:AGE-BSA可增加健康成人外周血单核细胞TNF-α的释放并可诱导健康人外周血单核细胞内HO-1mRNA的表达。结论:AGE可上调健康人外周血单核细胞TNF-α和细胞内HO-1mRNA的表达。Objective To investgate the effects of advanced glycation end-products （AGE）at different concentrations on the expressions of TNF-α and HO-1 in peripheral monocytes in healthy adults. Methods Peripheral monocytes were isolated,randomly divided into different groups,and then stimulated by AGE-BSA at different concentrations prepared in vitro. The level of TNF-α expression in the supernatant of cell culture and the intracellular HO-1 mRNA expression were detected. Results AGE-BSA enhanced the release of TNF-α from peripheral monocytes and induced intracellular HO-1 mRNA expression in monocytes in healthy adults. Conclusion AGE-BSA may upregulate the expressions of HO-1 mRNA and TNF-α in peripheral monocytes.

关 键 词：糖基化 肿瘤坏死因子Α 外周血单核细胞 血红素加氧酶-1 

分 类 号：R363[医药卫生—病理学]