P33^(ING1)和Survivin及Ki67蛋白在食管癌发生发展中的作用  

Role of P33^(ING1),survivin and Ki67 protein in carcinogenesis and development of esophageal carcinoma

作　　者：张安平 杨永珠[2] 黄维坤 苟云久[2] 

机构地区：[1]武警甘肃总队医院外二科,兰州730050 [2]兰州大学第二医院胸心外科

出　　处：《中国肿瘤临床与康复》2008年第4期306-309,共4页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的检测P33ING1、Survivin和Ki67蛋白在食管正常黏膜、单纯增生、不典型增生、浸润癌中的表达情况,分析这三种蛋白在食管癌不同病变区域中的表达及与食管癌病理特征的关系。方法采用免疫组化方法检测P33ING1、Survivin和Ki67蛋白在51例食管癌大体标本中的不同病变区域的表达。结果P33ING1蛋白从正常黏膜、单纯增生、不典型增生增生、浸润癌呈渐进性低表达,而Survivin和Ki67蛋白则呈渐进性高表达。三种蛋白在正常黏膜中的表达与不典型增生及浸润癌中的表达差异有显著性(P<0.01);P33ING1蛋白在食管癌中的表达与癌肿浸润深度(肌层至全层)呈负相关(P<0.05),与分化程度(G1、G3)呈正相关(P<0.05);Survivin蛋白表达与食管癌分化程度、淋巴结转移有关(P<0.05);P33ING1蛋白在浸润癌中的低表达与Survivin和Ki67蛋白高表达呈负相关(P<0.01)。结论Survivin功能的激活、Ki67增殖及P33ING1功能的下调可能共同对抗细胞凋亡,参与食管癌的发生发展。三者有可能为临床上食管癌早期诊断、相关治疗及预后研究提供新的手段。Objective To examine the expression P33^ING1, Survivin and Ki67 protein in different esophageal epithelia, including normal, hyperplasia, dysplasia and invasive carcinoma and to analyze the correlation of expression of P33^ING1 , Survivin and Ki67 with pathologic characteristics of esophageal carcinomas. Methods Immunohistochemistry was used to examine the expression of P33^ING1 , Survivin and Ki67 in 51 whole specimens of esophageal carcinoma with different degrees of epithelial lesions. Results The expression of P33^ING1 was reducing and that of Survivin and Ki67 was progressively increasing from normal epithelia, simple hyperplasia and dysplasia to invasive carcinoma. Significant differences were observed in the expression of P33^ING1 , Survivin and Ki67 between normal and dysplasia, invasive carcinoma （ P 〈 0. 01 ）. The expression of P33^ING1 was negatively correlated to the depth of invasion and was positively cerrelated to the degree of differentiation （ G1, G3 ） of the esophageal carcinomas （ P 〈 0.05 ）. The expression of P33^ING1 in esophageal invasive carcinoma was negatively correlated with that of Survivin and Ki67（ P 〈 0. 01 ）. Conclusion The activation of Survivin gene, enhancement of Ki67 function and down-regnlation of P33^ING1 function may jointly resist cell apoptosis and contribute to the carcinogenesis and development of esophageal carcinoma. They may provide new means for early diagnosis, therapy and prognosis of esophageal carcinomas.

关 键 词：食管肿瘤 P33^ING1 SURVIVIN KI67 

分 类 号：R735.1[医药卫生—肿瘤] R730.43[医药卫生—临床医学]