苯乙酸对胶质瘤C6细胞增殖和凋亡的影响  

Effect of phenylacetate on proliferation and apoptosis of rat glioma C6 cells

作　　者：杨朝华[1] 李小玉[2] 游潮[1] 李国平[1] 邓峰[3] 罗大山[3] 

机构地区：[1]四川大学华西医院神经外科 [2]四川大学口腔生物医学工程教育部重点实验室,四川成都610041 [3]广西北海市人民医院神经外科,广西北海536000

出　　处：《中华神经外科疾病研究杂志》2008年第4期298-301,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：广西科学基金(桂科青0640078)

摘　　要：目的观察诱导分化剂苯乙酸(PA)对胶质瘤C6细胞增殖和凋亡的影响。方法体外培养胶质瘤C6细胞,用0、2.5、5.0和7.5 mmol/L不同浓度的PA,分别在PA诱导24、48、72、96 h后,甲基噻唑基四唑(MTF)比色法检测细胞增殖抑制率,进一步用免疫细胞化学检测胶质纤维酸性蛋白(GFAP)的表达变化,末端脱氧核糖核酸转移酶介导的脱氧尿嘧啶(?)苷酸缺口末端标记(TUNEL)检测细胞凋亡。结果PA显著抑制C6细胞增殖,随药物浓度的增加阳作用时间的延长.细胞增殖抑制率增加。PA作用后GFAP表达增强。随PA浓度和作用时间的增加,细胞凋亡率增加:结论PA对胶质瘤C6细胞有显著的增殖抑制和诱导凋亡作用,呈时间剂量依赖性。Objective To observe the effect of phenylacetate （PA） on proliferation and apoptosis of rat glioma C6 cells. Methods C6 cells were cultured in vitro. After induced by PA, the rate of cell inhibition was measured by the methyl thiazolyl tetrazolium （MTF） assay, the expression of glial fibrillary acidic protein （GFAP） was determined by immunecytechemistry and the apoptosis was detected by terminal deoxynucleotide transferase-mediate deoxyuridine triphosphate nick end labeling （TUNEL） assay. Results The proliferation of C6 cells was obviously inhibited by PA. With the increase of PA concentration and treatment time, the rate of cell inhibition was increased. The expression of GFAP was enhanced by PA. The apoptosis rate was remarkably increased with the increase of PA concentration and treatment time. Conclusion PA can inhibit cell proliferation and induce apoptosis in C6 cells in a time- and dose-dependent manner.

关 键 词：苯乙酸 胶质细胞瘤 凋亡 

分 类 号：R730.264[医药卫生—肿瘤]