他莫昔芬对SHG-44胶质瘤细胞氯通道的抑制作用  

The block effect of tamoxifen on voltage-dependent chloride channel in SHG-44 glioma cell line

作　　者：王帅[1] 焦保华[1] 

机构地区：[1]河北医科大学第二医院神经外科,河北石家庄050000

出　　处：《中华神经外科疾病研究杂志》2008年第4期302-304,共3页Chinese Journal of Neurosurgical Disease Research

摘　　要：目的研究氯通道阻断剂他莫昔芬(tamoxifen)对SHG-44胶质瘤细胞电压依赖性氯通道电流的作用。方法采用全细胞膜片钳方法记录SHG-44细胞的电压依赖性氯通道电流,并观察施用不同浓度的他莫昔芬后电流的变化。结果该电流特性为外向整流、不失活,他莫昔芬能够明显阻断该电流,该阻断具有剂量依赖性及电压依赖性。在+100 mV时,μM及5μM他莫昔芬对氯电流抑制率分别为48%及89%。结论他莫昔芬可明显阻断SHC-44胶质瘤细胞上的电压依赖性氯通道。Objective To explore the effect of tamoxifen on voltage-dependent chloride channel in SHG-44 glioma cell line. Methods Whole-cell patch clamp technique was used to record the chloride currents in SHG- 44 cell line and to investigate the effect of tamoxifen of different concentration on this channel currents. Results This channel was outward-rectified and didn＇t inactivate. Tamoxifen could obviously decrease the amplitude of chloride currents of SHG-44 cell line. This block effect was dose- and voltage- dependent. With the holding potential of ＋ 100 mV, the block rate of 1μM and 5 μM tamoxifen could reach 48% and 89%. Conclusion Tamoxifen can significantly block the voltage-dependent chloride channel in malignant glioma cell line SHG-44.

关 键 词：SHG-44胶质瘤细胞 氯离子通道 膜片钳技术 

分 类 号：R730.23[医药卫生—肿瘤]