Beclin1和NF-κB p65在肝癌中的表达及其临床意义  

Expression and clinical significance of Beclin1 and NF-κB p65 protein in primary human hepatocellular carcinoma

作　　者：康凯夫[1] 王晓蔚[1] 陈小伍[1] 石祥呈[1] 

机构地区：[1]南方医科大学附属顺德第一人民医院病理科,广东省顺德市528300

出　　处：《世界华人消化杂志》2008年第20期2244-2247,共4页World Chinese Journal of Digestology

摘　　要：目的:分析自噬基因Beclin1和核因子NF-κB(nuclear factor-kappaB,NF-κB)在原发性肝细胞性肝癌(primary human hepatocellular carcinoma,HCC)中的表达及其临床意义.方法:收集顺德第一人民医院2003-01/2007-12手术切除经病理学证实的HCC50例,肝炎后肝硬化手术切除或活检标本30例,肝炎患者肝脏穿刺组织30例和正常肝组织10例.应用免疫组化S-P法检测肝脏组织中Beclin1和NF-κBp65蛋白的表达.结果:原发性肝细胞性肝癌、肝硬化、肝炎和正常肝组织的Beclin1蛋白阳性表达率分别是78.00%(39/50)、26.67%(8/30)、53.33%(16/30)、10.00%(1/10),四者之间的差异有统计学意义(χ2=28.31,P<0.05),且Beclin1在原发性肝细胞性肝癌组织表达明显高于在肝硬化组织,肝炎组织,正常肝组织中的表达(χ2=20.39,5.31,14.41,P<0.05);在肝炎组织表达明显高于在肝硬化组织,正常肝组织中的表达(χ2=4.44,χ2=4.12,P<0.05).原发性肝细胞性肝癌、肝硬化、肝炎和正常肝组织的NF-κBp65蛋白阳性表达率分别是74.00%(37/50)、36.67%(11/30)、30.00%(9/30)、20.00%(2/10),四者之间的差异有统计学意义(χ2=22.00,P<0.05),且NF-κBp65在原发性肝细胞性肝癌组织表达明显高于在肝硬化组织,肝炎组织,正常肝组织中的表达(χ2=10.89,χ2=14.85,χ2=8.44,P<0.05).结论:Beclin1和NF-κBp65在HCC中的表达关系密切,呈正相关;Beclin1和NF-κBp65异常表达与HCC的发展密切相关,在HCC的发展过程中起重要的作用.AIM： To explore the expression and clinic significance of Beclinl and Nuclear factorkappa B （NF-kB p65） protein in primary human hepatocellular carcinoma （HCC）. METHODS： Fifty pathologically-confirmed HCC samples, 30 post-hepatitis cirrhosis samples, 30 hepatitis tissue samples and 10 normal hepatic tissue samples from January 2003 to December 2007 were collected. The expressions of Beclinl and NF-kB p65 in liver tissues were detected using immunohistochemistry. RESULTS： The expressions rates of Beclinl protein in cancer tissues, cirrhosis tissues, hepatitis tissues, normal tissues were respectively 78.00%（39/50）, 26.67%（8/30）, 53.3%（16/30） and 10.00%（1/10）. The four were statistically differences among, the significant （2 = 28.31, P 〈 0.05）. The expressions of Beclinl protein was significantly higher in HCC tissues than in cirrhosis tissues, hepatitis tissues or normal tissues （x2 = 20.39, x2 = 5.31, x2 = 14.41, P 〈 0.05）. The expressions of Beclinl protein was markedly higher in hepatitis tissues than in cirrhosis tissues and normal tissues（x2= 4.44, x2= 4.12, P 〈 0.05）. The expressions rates of NF-KB p65 protein in HCC tissues, cirrhosis tissues, hepa- titis tissues and normal hepatic tissues were 74.00%（37/50）, 36.67%（11/30）, 30.0%（9/30） and 20.00%（2/10）, respectively. The differences among the four were statistically significant （x2= 22.00, P 〈 0.05）. The expression of NF-~：B p65 protein was markedly higher in HCC tissues than in cirrhosis tissues, hepatitis tissues and normal hepatic tissues（;（x2=10.89, x2= 14.85, x2 = 8.44, P 〈 0.05）. CONCLUSION： Beclinl expression is positively correlated with NF-kB p65 expression in HCC. Aberrant expressions of Beclinl and NF-kB p65 are closely related with HCC development. They may play important roles in HCC development.

关 键 词：BECLIN1 核因子ΚB 免疫组化 

分 类 号：R735.7[医药卫生—肿瘤] R692.3[医药卫生—临床医学]