hPTTG1在结直肠癌中的表达及其临床意义  

Expression of human pituitary tumor transforming gene 1 in colorectal cancer and its clinical signif icance

作　　者：吴斌文[1] 马东[2] 李友佳[1] 李东风[3] 邓罡[1] 曾志刚[1] 张凯军[1] 张瑛华[1] 耿庆山[1] 

机构地区：[1]广东省人民医院东病区消化内科,广东省老年医学研究所,广东省广州市510080 [2]广东省人民医院肿瘤中心肿瘤内科,广东省广州市510080 [3]广东省人民医院医学实验中心,广东省广州市510080

出　　处：《世界华人消化杂志》2008年第20期2248-2252,共5页World Chinese Journal of Digestology

摘　　要：目的:检测人垂体瘤转化基因1(hPTTG1)在结直肠癌中的表达,分析其与临床病理参数之间的关系,为阐明hPTTG1在结直肠癌诊断和转移复发中的临床价值提供实验依据.方法:收集我院2004-01/2006-09结直肠癌及对应癌旁组织的手术标本60例.采用免疫组织化学和Western blotting检测结直肠癌组织与对应癌旁组织hPTTG1蛋白的表达,并探讨其与临床病理指标的关系.结果:60例结直肠癌组织有56例表达hPTTG1蛋白,阳性率为93.3%,而癌旁组织hPTTG1仅8例表达,且均为弱阳性,阳性率为13.3%(χ2=77.13,P<0.001).hPTTG1表达与血清CEA水平具有显著相关,血清CEA大于5mg/L较小于5mg/L显著增高(χ2=30.886,P<0.001).hPTTG1在DukesC、D期显著高于DukesA、B期(χ2=9.87,P<0.001).伴有淋巴转移、肝转移或其他器官转移者较无转移者显著增高(χ2=9.87,P<0.001).hPTTG1与患者的性别、年龄、肿瘤直径大小和病理分型无关.结论:hPTTG1在结直肠癌中高表达,与结肠癌的疾病进展密切相关,检测hPTTG1有助于患者预后的判断.AIM： To investigated the expression of human pituitary tumor transforming gene 1 （hPTTG1） protein in colorectal cancerous tissues and to analyze its relationship with clinicopathological parameters so as to gain more experimental evidence for hPTTG1＇s clinical value in colorectal cancer diagnosis and relapse. METHODS： Sixty colorectal cancer samples and their corresponding noncancerous tissues were examined for hPTTG1 protein expression us- ing immunohistochemical staining and Western blotting analysis. Subsequently, we also studied the relationship between seven clinicopathologi- cal parameters and hTTG1 protein expression. RESULTS： Of 60 colorectal tissue samples, 50 samples of hPTTG1 protein expression were positive （93.3%）, but only 8 corresponding noncancerous tissues were positive and all weakly positive （13.3%） （X2 = 77.13, P 〈 0.001）. There was a significant correlation between hPTTG1 mRNA expression and serum CEA level, and CEA ≥ 5 mg/L was significantly higher than CEA 〈 5 mg/L （2 = 30.886, P 〈 0.001）; hPTTG1 gene was expressed significantly higher in Dukes C, D stages than in Dukes A, B stages （2 = 9.87, P 〈 0.001）, and significantly higher in colorectal cancer with the lymph node invasion, liver or other organs metastasis than no invasion （2 = 9.87, P 〈 0.001）. The hPTTG1 expression was not correlated with age, sex, tumor size or histologic types. CONCLUSION： The hPTTG1 is overexpressed in and closely correlated with the progress of colorectal cancer, therefore hPTTG1 expression may be helpful for the judgement of the prognosis for colorectal cancer patients.

关 键 词：结直肠癌 人垂体瘤转化基因1 转移 免疫组化 

分 类 号：R735.3[医药卫生—肿瘤]