慢性应激与氟西汀对大鼠海马结构与杏仁核神经营养因子蛋白与核酸表达的影响  

Differential regulation of neurotrophic factor and it receptor mRNA and protein by chronic fluoxetine and stress in the adult rat hippocampus and Amygdaloid nuclei

作　　者：郁镠宇 许珂[2] 孙学礼[2] 刘协和[2] 

机构地区：[1]广西医科大学第一附属医院心理卫生中心,530021 [2]四川大学华西医院心理卫生中心

出　　处：《四川精神卫生》2008年第3期132-136,共5页Sichuan Mental Health

摘　　要：目的观察慢性温和未预知应激抑郁模型及氟西汀对大鼠海马与杏仁核神经元BDNF/Trk与NT-3/TrkC表达的影响。方法将32只SD雄性大鼠随机均分为正常组、慢性应激模型组、氟西汀组与生理盐水组。选用慢性轻度不可预见性应激加孤养造模,分别采用免疫组织化学、定量逆转录聚合酶链反应检测慢性应激与氟西汀处理的大鼠海马与杏仁核BDNF/TrkB蛋白和信使核酸的表达。结果与其相应的对照组相比,两干预组海马海马回区(CA)与齿状核(DG)BDNF表达呈现相反的变化,而杏仁核的表达则相似。慢性应激组与氟西汀组NT-3在海马与齿状核受调节的方向迥异。TrkB与TrkC表达水平与其配体调节方向略有差别。结论慢性温和未预知应激与氟西汀对海马与杏仁核BDNF/Trk与NT-3/TrkC表达的不同调节方向,可能反映慢性应激抑郁模型动物的病理过程与氟西汀治疗效应之间的差异。Objective To observe the effect of chronic unpredicted sequence of mild stress （CUMS） and fluoxetine on the expression of brain - derived neurotrophic factor （BDNF）, neurotrophin - 3 （NT -3 ） and their functional receptors TrkB/trkC in the hippocampus and Amygdoid nuclei of adult rat. Methods Thirty - two male SD rats were randomly and equally allocated to 4 groups. Quantative reverse transcription polymerase chain reaction（ RT - PCR） and immunohistochemistry were used to examine the expression levels of BDNF/TrkB and NT - 3/TrkC protein and mRNA after CUMS and fluox- etine management. Results After the experiment, the opposite regulation of BDNF in the hippocampus and similar in the Amygdoid nuclei were shown to compare with their control groups, different expression direction of NT - 3 in the hippocampus and Amygdoid nuclei was presented. And changes in the pattern of their receptors TrkB/trkC differed from their corresponding ligand. Conclusion Different models of expression in CUMS and fluoxetine groups suggested a role for them in the onset of ant/depressive effectivity and the pathogenesis of depression.

关 键 词：慢性应激 氟西汀 神经营养因子 受体 海马 

分 类 号：R749.053[医药卫生—神经病学与精神病学]