血清中有机氯农药残留及谷胱甘肽转移酶T1基因多态性与乳腺癌关系的病例对照研究  

A case-control study on serum organochlorines residues, genetic polymorphisms of glutathione S-transferase T1 and the risks of breast cancer

作　　者：常永丽[1,4] 李君[1] 姚三巧[1] 胡万宁[2] 蒋守芳[1] 郭忠[3] 杨莉[1] 李丹丹[1] 李亚梅[1] 刘岩[1] 

机构地区：[1]华北煤炭医学院预防医学系,唐山063000 [2]唐山市肿瘤医院 [3]唐山市疾病预防控制中心 [4]邢台医学高等专科学校,054001

出　　处：《中华流行病学杂志》2008年第8期763-766,共4页Chinese Journal of Epidemiology

摘　　要：目的研究血清中有机氯农药水平及谷胱甘肽转移酶T1（GSTT1）基因多态性对女性乳腺癌患病风险的交互作用。方法运用1：1配比的病例对照研究方法，自2006年9月至2007年10月，收集经病理学确诊为乳腺癌的女性病例及对照共70对。采用问卷调查获取病例和对照乳腺癌相关危险因素的信息；采用气相色谱-电子捕获（GcECD）方法检测血清中有机氯农药残留水平，用PCR检测GSTT1基因多态性，根据交互作用系数（γ=βeg/βe）判断交互作用存在与否以及不同的基因-环境作用类型。结果GSTT1基因多态性与氯苯乙烷（DDT）及六氯环己烷（HCH）间存在一定的交互作用，交互作用系数分别为1．352、1．528，交互作用都表现为超相乘模型。结论乳腺癌的发生是环境和遗传因素综合作用的结果。GSTT1基因多态性与环境危险因素DDT／HCH的暴露在乳腺癌发生中存在一定的交互作用。Objective To study the potential effect of gene-environment interaction between glutathione S-transferase T1 （GSTT1） and serum organochlorines residues on the risk of breast cancer in women, in China. Methods 70 newly diagnosed female breast cancer patients and 30 controls from September 2006 to October 2007 were interviewed using the same questionnaire to obtain information regarding exposure to those risks. Organochlorine residues level in serum was measured by gas chromatography （GC）. Genotypes of GSTT1 polymorphisms were analyzed by multiplex allele-specific polymerase chain reaction （PCR）. Interaction indexes （γ） were calculated to determine the type of geneenvironment interaction. Results After adjusting the confounding factors, results showed that interaction existed in genetic polymorphisms of GSTT1 and dichlorodiphenyltrichloroethane （ DDT ）/ hexachlorocyclohexane （HCH） residues, with interaction indexes （γ） value as 1.352 and 1.528. Conclusion Genetic and environmental hazard factors had a co-effect on the development of breast cancer while genetic polymorphisms of GSTT1 and DDT/HCH expressed an interaction to breast cancer.

关 键 词：乳腺肿瘤 基因多态性 交互作用 氯苯乙烷 六氯环己烷 谷胱甘肽转移酶T1 

分 类 号：R737.9[医药卫生—肿瘤]